Topic
Zeatin
About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.
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01 Mar 2009
8 citations
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TL;DR: Improved performance of the nitrogen-phosphorus and flame-photometric detectors gave greatly enhanced sensitivity and-or specificity compared with the flame-ionisation detector for a wide range of cytokinin types was used to develop rapid and simple purification techniques.
Abstract: The performances of four gas chromatographic detectors (nitrogen-phosphorus, flamephotometric, electron-capture and flame-ionisation) were compared for the analysis of permethylated cytokinins. The nitrogen-phosphorus and flame-photometric detectors (selective for nitrogen and sulphur-containing compounds respectively) gave greatly enhanced sensitivity and-or specificity compared with the flame-ionisation detector for a wide range of cytokinin types. The response of the electron-capture and flame-ionisation detectors was quantitatively similar. The improved performance of the nitrogen-phosphorus and flame-photometric detectors was used to develop rapid and simple purification techniques for the assay of zeatin and zeatin riboside in coconut milk; dihydrozeatin and dihydrozeatin riboside in bean leaves and cytokinin nucleosides in wheat-germ transfer RNA.
8 citations
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TL;DR: Thidiazuron (TDZ) and zeatin were more effective than BA in producing adventitious shoots from A. deliciosa leaf and callus tissue and A. arguta tissues grown in vitro.
Abstract: Thidiazuron (TDZ) is a compound with cytokinin-like activity. Its activity was compared with that of benzyladenine (BA) and zeatin, either alone or in combination with naphthaleneacetic ocid (NAA), for adventitious shoot production in Actinidia deliciosa and A. arguta tissues grown.in vitro. TDZ and zeatin were more effective than BA in producing adventitious shoots from A. deliciosa leaf and callus tissue. High-quality shoots were formed from leaf explants of A. deliciosa using low concentrations of TDZ. A. arguta shoot regeneration was less successful. No shoots formed on BA media, although shoots formed on TDZ media (5–45 µM) in the presence of NAA and on media with 45 µM zeatin.
8 citations
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01 Jan 2021TL;DR: Evaluating the micro-propagation feasibility inside olive glasses of olive cultivar Kroneiki found the most effective culture medium and hormone on branching were MSM culture medium with 1 ppm BAP hormone and on rooting, MS1 culturemedium with 1.5 ppm B AP hormone and 0.75 ppm NAA hormone.
Abstract: Background: A long time has passed since the first experience of In Vitro propagation of olive, but the presence of very strong end dominance in the neoplasm stage, which cannot be controlled by various cytokinin treatments, has limited the possibility of propagation in vitro. Therefore, the aim of the present study was to evaluate the micro-propagation feasibility inside olive glasses of olive cultivar Kroneiki. Methods: For branching and rooting of lateral buds, the experiment was performed factorially in a completely randomized design with three replications. Branching factors include five types of culture media (MS, MS1/2, MSM, MSM1/2 and OM), two types of hormones (BAP and Zeatin), and three types of hormone concentrations (control, 0.5 and 1 mg/l) and there were three types of time periods. Rooting factors include five types of culture media (MS, MS1/2, MSM, MSM1/2 and OM), two types of hormone combinations (IBA and NAA), four types of hormone concentrations (control, combination of 0.2 with 0.5, combination 0.5 with 1 and a combination of 0.75 with 1.5 mg/l) and three types of time intervals. Analysis of variance was performed by Statistix10 software and the comparison of mean traits was performed using the least significant difference test. Results: The effect of culture medium, different hormones and also different concentrations of hormones used in the period, on the number of green leaves, number of yellow leaves, fresh weight and dry weight of seedlings as well as rooting were different (P <0.01). The highest length of branch (1.8 cm) was obtained in MSM culture medium with BAP hormone in the third week. The highest fresh weight (0.06 g), the highest dry weight (0.003 g) and the highest amount of green leaves (55.39%) were obtained from the treatment of MSM medium with 1 ppm of BAP hormone. The highest rooting rate (95.17%) was obtained from the treatment of MS1/2 medium with 1.5 ppm of BAP hormone and 0.75 ppm of NAA hormone in the third week. Conclusion: The most effective culture medium and hormone on branching were MSM culture medium with 1 ppm BAP hormone and on rooting, MS1 culture medium with 1.5 ppm BAP hormone and 0.75 ppm NAA hormone were obtained, respectively.
8 citations
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TL;DR: The lack of response to application of exogenous zeatin or 6-benzylaminopurine, together with the data on endogenous cytokinins, suggests that the level of cytokinin bases and nucleosides was not limiting the growth rate of the Norwegian population in SD at 8°C.
Abstract: SUMMARY
The effects of photoperiod (8 or 16 hr, SD or LD) and temperature (low or high, 8° or 20°C) on the growth and cytokinin content of Norwegian and Portuguese populations of Dactylis glomerata (Cocksfoot) were investigated.
In general, conditions which promote active leaf growth (LD or high temperature) of plants of the Norwegian population tend to result in lower levels of cytokinin bases and/or nucleosides and in higher levels of ‘nucleotide’ cytokinins than conditions which lead to reduced growth rate (SD or low temperature). In some ‘transfer’ experiments the changes in hormone levels preceded detectable changes in growth rate. Cytokinins which behaved similarly to zeatin and zeatin riboside on Sephadex LH 20 columns were detected.
The lack of response to application of exogenous zeatin or 6-benzylaminopurine, together with the data on endogenous cytokinins, suggests that the level of cytokinin bases and nucleosides was not limiting the growth rate of the Norwegian population in SD at 8°C. However, if ‘nucleotide’ cytokinins are significant for growth rate under unfavourable conditions then this class of hormone may be involved in the responses to day length and temperature.
8 citations