Topic
Zeatin
About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.
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TL;DR: The emergence of roots from callus of recalcitrant Ugandan cultivars and the comparable high embryogenic responses in this work demonstrate the potential for regenerating plants from African cultivars that have not been regenerated before.
Abstract: Genetic transformation is considered as one of the most promising options for improvement of crop traits Current transformation methods for sweetpotato depend on plant regeneration through organogenesis or somatic embryogenesis Somatic embryogenesis and plant regeneration at a high frequency has been restricted to a few sweetpotato varieties Three auxins namely: 2,4-dichlorophenoxyacetic acid (2,4-D), 4-fluoroamphetamine (4-FA) and 4,5-trichlorophenoxyacetic acid (2,4,5-T) were investigated in this study for enhancing somatic embryogenesis from various plant organs of recalcitrant African sweetpotato cultivars 2,4-D was found to be the best (p 005) for induction of embryogenic callus Cultivar Bwanjule had the highest (202%) embryogenic callus frequency among the five African cultivars tested The highest number of plants in this study was regenerated from the non-African cultivar variety Jonathan on media supplemented with 02 mg Zeatin The emergence of roots from callus of recalcitrant Ugandan cultivars and the comparable high embryogenic responses in this work demonstrate the potential for regenerating plants from African cultivars that have not been regenerated before The regeneration of roots in this work could be useful for the initiation of root cultures The most important application of this work is in genetic transformation of sweet potato, particularly for improvement of resistance to weevils Key words : Embryogenesis, plant growth regulators, plant regeneration, Ipomoea batatas
7 citations
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TL;DR: It was concluded that there was a great variation on endogenous plant hormones among the annual wild Cicer species and the data may support selection for various attributes.
Abstract: All processes that regulate of growth, differentiation and development and also stomatal movement are influenced by endogenous hormones in plants. Research related with endogenous hormones is known for cultivated chickpea (Cicer arietinum L.), while in wild chickpeas no data existed. In this study, C. bijugum K.H. Rech.; C. chorassanicum (Bge) M. Pop.; C. cuneatum Hochst. ex Rich.; C. echinospermum P.H. Davis; C. judaicum Boiss.; C. pinnatifidum Jaub. et Sp.; C. reticulatum Ladiz.; and C. yamashitae Kitamura were evaluated for variations in endogenous plant hormone concentrations; indole-3-acetic acid, zeatin, gibberellic acid, and abscisic acid in both leaf and pod. It was concluded that there was a great variation on endogenous plant hormones among the annual wild Cicer species. The data may support selection for various attributes.
7 citations
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TL;DR: Protoplasts from leaves of regenerated shoots cultured in vitro divided and formed callus which again and reliably produced shoots and plantlets when placed on MS-media as specified before.
7 citations
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TL;DR: Ascisic acid enhances MaMADS2 expression to promote ripening, but the transcription factor in turn auto inhibits ABA synthesis and signaling, demonstrating a complex interaction of plant hormones and banana fruit ripening mediated by MaM ADS2.
Abstract: While the role of ethylene in fruit ripening has been widely studied, the contributions of additional plant hormones are less clear. Here we examined the interactions between the transcription factor MaMADS2-box which plays a major role in banana fruit ripening and hormonal regulation. Specifically, we used MaMADS2 repressed lines in transcriptome and hormonal analyses throughout ripening and assessed hormone and gene expression perturbations as compared to wild-type (WT) control fruit. Our analyses revealed major differences in hormones levels and in expression of hormone synthesis and signaling genes mediated by MaMADS2 especially in preclimacteric pulp. Genes encoding ethylene biosynthesis enzymes had lower expression in the pulp of the repressed lines, consistent with reduced ethylene production. Generally, the expression of other hormone (auxin, gibberellins, abscisic acid, jasmonic acid and salicylic acid) response pathway genes were down regulated in the WT pulp prior to ripening, but remained high in MaMADS2 repressed lines. Hormone levels of abscisic acid were also higher, however, active gibberellin levels were lower and auxin levels were similar with MaMADS2 repression as compared to WT. Although abscisic level was higher in MaMADS2 repression, exogenous abscisic acid shortened the time to ethylene production and increased MaMADS2 mRNA accumulation in WT. Exogenous ethylene did not influence abscisic acid level. CRE - a cytokinin receptor, increased its expression during maturation in WT and was lower especially at prebreaker in the repressed line and zeatin level was lower at mature green of the repressed line in comparison to WT. In addition to previously reported effects of MaMADS2 on ethylene, this transcription factor also influences other plant hormones, particularly at the pre-climacteric stage. The cytokinin pathway may play a previously unanticipated role via MaMADS2 in banana ripening. Finally, abscisic acid enhances MaMADS2 expression to promote ripening, but the transcription factor in turn auto inhibits ABA synthesis and signaling. Together, these results demonstrate a complex interaction of plant hormones and banana fruit ripening mediated by MaMADS2.
7 citations
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TL;DR: It was proved that Plantago maritima species was amenable to propagation from different kinds of explants and may be of significance for protection of sea plantain.
Abstract: A vanishing species in Poland - Plantago maritima L. was regenerated in vitro from tips of shoots (obtained in vitro) and from different explants of 4-week-old seedlings: seedling tips, hypocotyls, cotyledons, roots. Murashige and Skoog basal medium, supplemented with 0.6 pM indole-3-acetic acid in combination with cytokinins 6-benzyladenine, zeatin or kinetin, was used. The plants obtained in the result of micropropagation were normal in appearence. It was proved that Plantago maritima species was amenable to propagation from different kinds of explants. The method may be of significance for protection of sea plantain.
7 citations