Topic
Zeatin
About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.
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TL;DR: It is suggested that the increased supply of new wall material could be due to either a direct or indirect activation of the membrane-associated polysaccharide synthetases.
6 citations
TL;DR: The content of cytokinins and the activity of GA were much higher under LD conditions, especially in leaves, and the total level of both phytohormones was higher in transformed as compared to wild-type plants.
Abstract: We studied photoperiodic and hormonal regulation of tuberization in wild-type potato (Solanum tuberosum L., cv, Desiree) plants and derivative transgenic plants harboring the PHYB gene from Arabidopsis, which encodes the phytochrome B apoprotein, under the control of the cauliflower mosaic virus 35S promoter. Plants were cultured on hormone-free Murashige and Skoog nutrient medium containing 5% sucrose or on the same medium supplemented with 1 mg/l kinetin under conditions of long day (LD, 16 h), short day (SD, 10 h), or SD with interrupted long night. We estimated cytokinins (zeatin and zeatin riboside) in underground and aboveground plant organs by the ELISA technique and GA activity in a bioassay with dwarf pea seedlings. Under LD conditions, transgenic plants produced substantially less tubers than wild-type plants. Kinetin addition to the culturing medium resulted in stimulation of tuberization under LD conditions, especially pronounced in the PHYB plants. The content of cytokinins and the activity of GA were much higher under LD conditions, especially in leaves. The total level of both phytohormones was higher in transformed as compared to wild-type plants. A relation of phytochrome-dependent tuberization to the hormonal status of underground and above-ground plant organs and possible reasons for kinetin stimulatory effect on this process are discussed.
6 citations
TL;DR: Using solvent partitioning, partition chromatography on Sephadex LH-20 and mass spectrometry the major cytokinin present in malt extract was identified as zeatin this paper.
6 citations
TL;DR: The results suggest that the meristematic nodule is a promising material for propagation and long-term conservation of this plant.
Abstract: Multiple meristematic nodules were induced on leaf segments from the Japanese persimmon (Diospyros kaki Thunb.) ‘Fuyu’ that were cultured on a solidified half-strength Murashige and Skoog’s medium containing 2% (w/v) sucrose and 1 mM thidiazuron (TDZ). The nodules 1 to 3 mm in diameter were formed in the cut ends and abaxial side of the leaf segments two weeks after inoculation. The nodule changed from pale green to dense green and multiplied to form many daughter nodules. Adventitious buds were thereafter formed on the nodules, suggesting that the meristematic nodule is an intermediate structure for bud differentiation. The adventitious buds grew poorly on the medium with TDZ, but resumed growth and developed into shoots after transfer to the medium containing 2% (w/v) sucrose and 10 mM zeatin. The meristematic nodules survived more than one year on the medium containing 2% (w/v) sucrose and 1 mM TDZ without transfer, and did not lose their ability to form adventitious buds for more than 5 years when transferred regularly to a fresh medium. These results suggest that the meristematic nodule is a promising material for propagation and long-term conservation of this plant.
6 citations
TL;DR: An attempt to enhance transformation efficiency by optimizing infection time, co-cultivation period and in vitro regeneration system for insect resistant transgenic pea plants through Agrobacterium-mediated transformation.
Abstract: Pea is one of the most important legume crops whose production is constantly threatened by field and storage pests and diseases Developing insect resistant transgenic pea plants through Agrobacterium-mediated transformation is a promising solution to maintain crop yield However, the transformation efficiency is still low Therefore, there was an attempt to enhance transformation efficiency by optimizing infection time, co-cultivation period and in vitro regeneration system Transformation was performed using segments of embryonic axes from mature pea seeds (Pisum sativum L cv Sponsor) The segments were inoculated with the hyper virulent EHA105 strain of Agrobacterium tumefaciens The Agrobacterium strain harboured a binary vector pGII35S containing the bar gene which confers resistance to phosphinotricin The selection medium contained P2 medium with increasing concentrations of phosphinothricin To improve the regeneration efficiency, 45 μM zeatin was added to the selection medium The highest transformation efficiency (789%) was achieved with infection time of 90 min and co-cultivation period of 2 days The shoots elongated well and the number of shoots/explants was increased (6 folds) after addition of zeatin Resistant shoots were grafted onto rootstocks in soil and grafting success rate was 100% The integration of cry1Ac gene in T0 transgenic plants was confirmed primarily by Polymerase Chain Reaction and further analysed by Southern blotting
6 citations