Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Regeneration of plants from leaves of Chrysanthemum morifolium Ram. cv. Bronze Bornholm in in vitro cultures

Abstract: Plants were obtained from cultured in vitro leaves of Chrysanthemum morifolium Ram. cv. Bronze Bornholm. The leaves were inoculated on Murashige and Skoog medium (MS) supplemented with cytokinins (kinetin - KIN, zeatin - ZEA, 6-benzyloaminopurine - BAP) and auxins (2,4-dichlorophenoxyacetic acid - 2,4-D, α-naphtaleneacetic acid - NAA, 3-indolilacetic acid - IAA, p-fluorophenylalanine - PFA) in various combinations and concentra-tions. The most suitable medium was that one which contained 4 mg/l KIN, 2 mg/l NAA and 50 mg/l PFA.

Micropropagation of a Rare Tree Species, Empetrum nigrum var. japonicum K. Koch via Axillary Bud Culture

Abstract: In order to develop an efficient micropropagation system for a rare tree species, Empetrum nigrum var. japonicum K. Koch, the effect of medium salt, cytokinins and auxin at different concentration were evaluated. Shoot induction from axillary bud was better on WPM medium than on MS medium. Although there was no significant differences observed in shoot induction among the salt strengths of WPM medium, whereas healthy shoots were developed on basal WPM medium. In comparison of the cytokinins affecting shoot proliferation, zeatin was better than BA, whereas BA exhibited more effectiveness on shoot elongation. In vitro root formation was better on WPM medium than on 1/2MS medium and achieved the highest rooting rate when 5.0 mg/L IBA treatment. 93% of rooted plantlets were survived on artificial soil mixture after 4 weeks of acclimatization. Above results suggest that a rare tree species, E. nigrum var. japonicum can be micropropagated via axillary bud cultures.

Effect of fruit cryopreservation on in vitro germination of zygotic embryos of several species of ilex

Abstract: This report presents preliminary results from a study aimed to develop a protocol for fruit cryopreservation and the recovering of plants through zygotic embryo culture, as a tool for the conservation of Ilex spp. (Aquifoliaceae) genetic resources. Fruits from eight plant species were harvested and maintained at 4°C for 1 month. Direct immersion of fruits in liquid nitrogen was lethal to the embryos of all the plant species tested. However, the embryos of five species (Ilex brasiliensis, L dumosa, I. microdonta, I. paraguariensis and I. pseudoboxus) dissected from fruits (with a cryoprotective solution containing 50% sucrose and 50% glycerol), cryopreserved using a slow freezing method (1°C.min -1 until -40°C) and stored in liquid nitrogen, when cultured in vitro, germinated with frequencies ranging from 3 to 23%. The embryos were cultured in vitro in darkness using a culture medium composed of 1/4-strength Murashige and Skoog medium, with 3% sucrose, 0.65% agar and 0.1 mg L -1 zeatin. The germinated embryos developed into normal plantlets after 60 days in culture. These results suggest that this methodology might be used for long-term germplasm storage of Ilex species.

Phytohormones, Rhizobium Mutants, and Nodulation in Legumes. VI. Metabolism of Zeatin Riboside Applied Via the Tips of Nodulated Pea Roots

Abstract: [3H]zeatin riboside was supplied in physiological quantities to pea (Pisum sativum L. cv Greenfeast) plants by replacing the root tip with a small vial containing [3H]zeatin riboside, to simulate the normal supply of cytokinin. Radioactivity was transported to the root nodules. Analysis by two-dimensional thin layer chromatography revealed that little3H remained as zeatin riboside in root or nodule tissue at the end of the labeling period (2, 5, or 8 d) and suggested that the following compounds were metabolites of [3H]zeatin riboside: zeatin, adenosine, adenine, the O-glucosides of zeatin and zeatin riboside, nucleotides of adenine and zeatin, and the dihydro-derivatives of many of these compounds.