Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Comparison of endogenous cytokinins, ABA and metabolites during female cone bud differentiation in low and high cone-producing genotypes of lodgepole pine

Abstract: In lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia Engelm.), cone bud initiation within long-shoot buds varies according to genotype. We chose to study hormone profiles of two genotypes that differed significantly in cone yield. Phytohormone profiles were established by high performance liquid chromatography–electrospray ionization tandem mass spectrometry in multiple reaction monitoring mode with samples collected from genotypes 299 and 233, the typically high and low cone producers. Generally, concentrations of trans-zeatin-O-glucoside were higher in genotype 299, whereas dihydrozeatin concentrations were higher in genotype 233. Both isopentenyl adenine and isopentenyl adenosine were present at higher concentrations in genotype 233. The ratio of total quantifiable zeatin (Z)-type cytokinins to isopentenyl (iP)-type cytokinins was approximately threefold higher in genotype 299 during female cone bud differentiation. In genotype 299, ABA concentration was significantly lower than in genotype 233 on the first sampling date, while the phaseic acid concentration was lower consistently throughout the period investigated. Dihydrophaseic acid was present in low concentrations in most samples of genotype 233, but was not quantifiable in genotype 299. Our study reveals that long-shoot buds of the high cone-producing genotype had higher ratios of Z-type cytokinins to iP-type cytokinins than were found in the low cone-producing genotype. High cone-producing buds also contained less ABA, phaseic acid and dihydrophaseic acid during female cone bud differentiation.

A wound inducible cytochrome P450 from tomato

Abstract: cDNA clone sharing high similarity to Nicotiana plumbaginifolia putative cytochrome P450 monooxygenase was isolated from Lycopersicon esculentum cv. ‘Rutgers’. Isolated cDNA has a full open reading frame and its protein sequence identity to Nicotiana. plumbaginifolia P450 (CYP72A2) evaluated at 75%. Tomato genomic P450 sequence contains 3 short introns. Expression on mRNA level of tomato P450 was checked. The highest level of tomato P450 transcript was observed in young tissues. The transcript level of P450 was increased after mechanical wounding in tomato leaves. Zeatin spray and zeatin uptake do not affect tomato P450 expression.

In vitro plantlet production of the endangered Pinguicula vulgaris

Abstract: This study describes the development of a micropropagation protocol for Pinguicula vulgaris using cultures initiated from in vitro produced seedlings. P. vulgaris is a carnivorous plant with a northern, disjunctly circumpolar distribution and specific habitat requirements, and is hence becoming increasingly rare. Shoot proliferation was significantly influenced by Murashige and Skoog (MS) macronutrient concentration, showing higher proliferation rates in 1/4MS, but was not affected by the addition of 0.1 mg/L 6-benzyladenine (BA) or zeatin (Zea). The best medium for propagating P. vulgaris was plant growth regulator (PGR) free ¼MS. An average of 7.62 new shoots per initial explant could be obtained after 8 weeks of culture, of which over 79% produced roots during proliferation. Moreover, rooting percentages of 100% were obtained for the initial explants in all the tested media, including media without PGRs. The plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development.

Effects of Different Growth Regulators on the Rooting of Catalpa bignonioides Softwood Cuttings

Abstract: (1) Background: To further improve the rapid reproduction and large-scale application of Catalpa bignonioides. (2) Methods: With young softwood cuttings from a 3-year-old C. bignonioides mother plant used as materials, the effects of indole-3-acetic acid(IAA), indolebutyric acid(IBA) and rhizogenic powder-1(ABT-1) growth regulators at different concentrations on cutting indexes and the dynamic changes in endogenous hormone contents during the rooting of the C. bignonioides cuttings were studied. (3) Results: The rooting of C. bignonioides cuttings could be divided into five stages. There were three types of rooting of adventitious roots. IBA treatment resulted in a high rooting rate and beneficial root morphology. The morphological indexes of the cutting roots after treatment with 1000 mg·L−1 IBA had the best overall quality, which was significantly higher than that of the roots in the control (CK) group (p < 0.05). Although the average longest root length (20.51 cm) under ABT-1 was the longest, its overall average rooting rate was slightly lower than that under IBA. The rooting effect under IAA was generally lower than that under IBA and ABT-1. The endogenous hormone content of the cuttings was found to be closely related to rooting; the IAA and zeatin nucleoside (ZR) content was high, and the ratios of IAA/ABA and IAA/ZR were high. The contents of gibberellin3 (GA3) and abscisic acid (ABA) were low, which had a promoting effect on the rooting of the cuttings. (5) Conclusions: All three kinds of auxin can promote rooting and, of the three treatment groups, the rooting effect of cuttings in the IBA treatment group was the strongest, with 1000 mg·L−1 being the optimum concentration.

Effective Protocol for Agrobacterium-mediated Leaf Disc Transformation in Tomato (Lycopersicon esculetum Mill.)1jf

Abstract: An eflicient transformation system for Indian tomato cultivar, 'Pusa Ruby' was developed by identifying and optimizing various parameters affecting transformation rates, non-transformed growth and bacterial over growth. A protocol consisting leaf explants from 14-day-old in vitro seedlings, 2 day pre-conditioning on culture medium, 2 day co-cultivation period for inoculated explants, lOO ~m rl Kanamycin Sulphate and culture medium containing MS salt with 0.1 mg I-I IAA, 1.0 mg I-I Zeatin, 2% (w/v) sucrose,pH 5.8 resulted in significant higher transformation rate (66.7%) within 4-6 weeks period. Confirmation of transformation was carried out in protoplast, cell, tissue and shoot cultures by Kanamycin resistance; nopaline assay, NPT-II assay and PCR analysis with primers specific to IIpt-1l gene. This system will be certainly exploitable for transferring agronomically useful genes in tomato cultivars.