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Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.


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Journal ArticleDOI
TL;DR: In this article, an 8-week-old L. pumila var. alata nodal segment and leaf explants were cultured onto Murashige and Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, and 6-benzylaminopurine (BAP)) for shoot multiplication and auxins (2,4-dichlorophenoxyacetic acid (2-4-D) and picloram) for callus induction, respectively.
Abstract: This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old L. pumila var. alata nodal segment and leaf explants were cultured onto Murashige and Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, and 6-benzylaminopurine (BAP)) for shoot multiplication and auxins (2,4-dichlorophenoxyacetic acid (2,4-D) and picloram) for callus induction, respectively. The results showed that 2 mg/L zeatin produced the optimal results for shoot and leaf development, and 0.5 mg/L 2,4-D produced the highest callus induction results (60%). After this, 0.5 mg/L 2,4-D was combined with 0.25 mg/L cytokinins and supplemented to the MS medium. The optimal results for callus induction (100%) with yellowish to greenish and compact texture were obtained using 0.5 mg/L 2,4-D combined with 0.25 mg/L zeatin. Leaves obtained from in vitro plantlets and wild plants as well as callus were extracted and analyzed for their antioxidant activities (DPPH and FRAP methods) and polyphenolic properties (total flavonoid and total phenolic content). When compared with leaf extracts of in vitro plantlets and wild plants of L. pumila var. alata, the callus extract displayed significantly higher antioxidant activities and total phenolic and flavonoid content. Hence, callus culture potentially can be adapted for antioxidant and polyphenolic production to satisfy pharmaceutical and nutraceutical needs while conserving wild L. pumila var. alata.

5 citations

Book ChapterDOI
01 Jan 2001
TL;DR: It is suggested that NO3− application rapidly enhances root to shoot translocation of Z+ZR, thereby increasing Z+ zeatin+zeatin riboside concentrations in the leaf tissue, which finally stimulate leaf expansion.
Abstract: Hormonal effects have been implicated in rapid responses of plant growth, induced by application of different nitrogen (N) forms or different levels of N fertiliser supply. In this study, long- and short-term effects of different N forms on growth responses and cytokinin (CK) levels in root and leaf tissues and in xylem sap of tomato were investigated. Plants were cultivated in a pH-buffered nutrient solution (pH∼7), containing either NO3− or NH4+ [2 mM] as sole N source. Long-term treatment (7 d) with NH4+ decreased plant growth and particularly leaf expansion. This was associated with decreased zeatin+zeatin riboside (Z+ZR) concentrations in roots, xylem sap and leaves. In short-term experiments with NH4+ preculture, increased leaf expansion was detectable already 12 h after re-application of NO3− and coincided with enhanced Z+ZR levels in the xylem sap, in the leaf tissue but not in roots. The results suggest that NO3− application rapidly enhances root to shoot translocation of Z+ZR, thereby increasing Z+ZR concentrations in the leaf tissue, which finally stimulate leaf expansion.

5 citations

Journal ArticleDOI
TL;DR: In this paper, the first attempt of simultaneous separation of zeatin and kinetin using a simple C18 solid phase extraction (SPE) method coupled with ultra performance liquid chromatography (UPLC) was described.
Abstract: Trans-zeatin and kinetin are two cytokinin family members that exhibit an anti-aging effect on human fibroblast cells. These cytokinins can be found in coconut water and in the xylem sap of most plants. This study describes the first attempt of a simultaneous separation of zeatin and kinetin using a simple C18 solid phase extraction (SPE) method coupled with ultra performance liquid chromatography (UPLC). This method provided high recoveries of 95.2 and 98.6% for trans-zeatin and kinetin, respectively. Malayan Green Dwarf, a local coconut variety, was found to contain 14 μg/L trans-zeatin and 4 μg/L kinetin. This method will be useful for comprehensive research on different coconut varieties and determining the contents of cytokinins in coconut water.

5 citations

Journal ArticleDOI
01 Nov 2015-Bothalia
TL;DR: In this paper, the authors evaluated the effect of different combinations of the plant growth regulators 2,4-dichlorophenoxyacetic acid (2.4-D), indole-3-acetic acids (IAA), IBA, 6-(gamma, gamma-dimethylally-lamino) purine (2iP) and Zeatin (Zeatin) on the induction and development of somatic embryos.
Abstract: Stevia rebaudiana (Asteraceae) is a plant of economic importance because of its medicinal properties and the presence of sweetener compounds on its leaves. These compounds can be a substitute for sucrose in a wide variety of products used by persons with diabetes and obesity problems. To standardize an efficient and effective propagation method for the different Stevia genotypes grown in Colombia, this study evaluated the effect of different combinations of the plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 6-(gamma, gamma-dimethylallylamino) purine (2iP) and Zeatin on the induction and development of somatic embryos. Adenine and coconut water were also evaluated as supplements in the basal culture medium Murashige and Skoog Basal Salt Mixture (MS) with glutamine. The combination of 2,4-D (18.09 µM) and 2iP (7.38 µM) produced the highest number of somatic embryos per explant, which had well-defined characteristics. The genotype showed a significant effect on the embryogenic response. In the "SRQ-93" genotype, the formation and development of somatic embryos was achieved, whereas the genotypes "Bertoni" and "Morita II" only yielded embryogenic and non-embryogenic calli, respectively. The conversion to seedlings was achieved on the regeneration medium containing gibberellic acid (GA3) (0.29 µM) and activated charcoal.

5 citations

Journal ArticleDOI
TL;DR: The metabolism of [8- 14 C]zeatin was studied in attached and detached, seeded and induced parthenocarpic fruits of tomato, and in isolated seeds and fruit flesh, over the first 17 days of development.

5 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202333
2022103
202135
202034
201932
201848