Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Factors influencing plant regeneration from seedling explants of Hairy nightshade ( Solanum sarrachoides )

Abstract: Hairy nightshade (Solanum sarrachoides) has the potential to be a model system for the study of plant-pathogen interactions, however, the availability of tissue culture and transformation methods would strengthen its utility. For the development of tissue culture methods, we investigated, explant type (cotyledons, hypocotyls, roots), hypocotyl explant origin, cotyledon orientation (abaxial vs. adaxial) in direct contact with the medium, gelling agents (agar and agargel) and cytokinins (zeatin and 6-benzyladenine) at different concentrations. Cotyledon explants resulted in the greatest biomass as compared to root and hypocotyl. As for hypocotyl explant origin, explants proximal to the cotyledons had a significant effect on plant regeneration. However, cotyledon orientation and gelling agent had no effect on plant regeneration. Medium supplemented with either zeatin or 6-benzyladenine at 1 mg L−1 resulted in significant shoot regeneration. Shoots rooted readily when cultured on a non-hormone based rooting medium.

In vitro regeneration of Nicotiana africana plants from explants of different type and mesophyll protoplasts

Abstract: Methods of in vitro cultivation of an African endemic species Nicotiana africana (Solanaceae) possessing some valuable traits have been elaborated. Influence of different concentrations of auxin (alpha-naphtaleneacetic acid) and cytokinins (6-benzylaminopurine, zeatin, thidiazuron) on morphogenesis and plant regeneration has been analysed using leaves and internodes as explants. The optimum method of regeneration of N. africana shoots in leaf explants is cultivation in the presence of 0.1 mg/l NAA and 1 mg/l BA; in internode explants--cultivation on the medium containing 0.5 mg/l NAA and 1 mg/l zeatin. The use of thidiazuron was the most effective at the concentration of 0.4 mg/l with subsequent transfer of explants to hormone-free medium. Method of isolation and culture of mesophyll protoplasts enabling to regenerate N. africana plants during 3-4 months has been proposed.

Plant Regeneration from Mature Embryos of Endangered Species Pinus armandii Franch. var. amamiana (Koidz.) Hatusima.

Abstract: Mature embryos were excised from the seeds of Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endangered species only inhabiting the south west islands of Japan. They were cultured in vitro under different tissue culture conditions. Adventitious buds were induced on the surface of the embryo on medium containing BAP and they grew to shoots after subculturing to medium containing activated charcoal or a low concentration of thidiazuron. From the elongated shoots, root primordia and roots were induced in medium containing IBA as an auxine. We found that a low concentration of zeatin or BAP added to the medium was beneficial for plant regeneration of mature embryos of this species. There were many abnormal chlorophyll germinants from seeds collected in an isolated tree.

Effects of zeatin and activated charcoal in proliferation of shoots and direct regeneration in cotton (Gossypium hirsutum L.)

Abstract: A multiple shoot induction protocol was optimized for cotton (Gossypium hirsutumL.). Three cultivars of cotton (Sahel, Siokra, Hybrid, that is, Sahel × Siokra) were used to study the effects of zeatin and activated charcoal on proliferation of shoots and direct regeneration from shoot tip explant excised from 10 – 15 day-old seedlings cultured in vitro. Growth response of different varieties varied. Root and shoot formation was observed in all varieties. The best treatment for multiple shoot induction in cultivars was the treatment containing Murashig and Skoog (MS) basal medium supplemented with zeatin (0.1 mg/l) and activated charcoal (0.5 mg/l), while treatment containing zeatin (0.1 mg/l) and activated charcoal (2 mg/l) was not good medium for regeneration. Culture of every cultivar continued for 7 subcultures and morphological characteristic was evaluated during every subculture. Maximum length of shoots (4.65 cm), the highest percentage of root development (55%) and maximum number of nodes (6.7) was observed in Siokra variety in the second subculture, Hybrid genotype and siokra in the last subculture, respectively. Also the shape of leaves changed in Siokra variety during subcultures. Key words: Gossypium hirsutum L., regeneration, shoot tip culture, zeatin.

The metabolism of 6-(2,3,4-trihydroxy-3-methylbutylamino) purine by soybean callus

Abstract: 6-(2,3,4-trihydroxy-3-methylbutylamino) purine (trihydroxyzeatin) applied to soybean callus is metabolised slowly. After 48 h only one peak of biological activity which co-eluted with the applied cytokinin was detected. When the callus was incubated on a medium which contained 10−5 M trihydroxyzeatin, spiked with 8 {14C} trihydroxyzeatin, for 28 days, three peaks of biological activity and three peaks of radioactivity were detected. One of the biologically active and radioactive peaks co-eluted with zeatin. Another of the radioactive peaks co-eluted with N-(purin-6-yl) glycine. From the data obtained it apears that trihydroxyzeatin can be both oxidized and reduced by soybean callus. The potential to be converted to zeatin may explain why trihydroxyzeatin and its parent compound, which is usually rapidly metabolised by living material, are equally active in the soybean callus bioassay. From the radioactive data obtained it appears that trihydroxyzeatin is susceptible to oxidation to form N-(purin-6-yl) glycine.