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Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.


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Journal ArticleDOI
TL;DR: In this article , Naphthalene acetic acid (NAA) was applied on the stem bases of green and red-stem cuttings of 'Echa 1 hao' tea plant.
Abstract: Tea plant breeding or cultivation mainly involves propagation via cuttings, which not only ensures the inheritance of the excellent characteristics of the mother plant but also facilitates mechanized management. The formation of adventitious root (AR) determines the success of cutting-based propagation, and auxin is an essential factor involved in this process. To understand the molecular mechanism underlying AR formation in nodal tea cuttings, transcriptome and endogenous hormone analysis was performed on the stem bases of red (mature)- and green (immature)-stem cuttings of 'Echa 1 hao' tea plant as affected by a pulse treatment with naphthalene acetic acid (NAA).In this study, NAA significantly promoted AR formation in both red- and green-stem cuttings but slightly reduced callus formation. External application of NAA reduced the levels of endogenous indole-3-acetic acid (IAA) and cytokinin (TZR, trans-zeatin riboside). The number of DEGs (NAA vs. CK) identified in the green-stem cuttings was significantly higher than that in the red-stem cuttings, which corresponded to a higher rooting rate of green-stem cuttings under the NAA treatment. A total of 82 common DEGs were identified as being hormone-related and involved in the auxin, cytokinin, abscisic acid, ethylene, salicylic acid, brassinosteroid, and jasmonic acid pathways. The negative regulation of NAA-induced IAA and GH3 genes may explain the decrease of endogenous IAA. NAA reduced endogenous cytokinin levels and further downregulated the expression of cytokinin signalling-related genes. By the use of weighted gene co-expression network analysis (WGCNA), several hub genes, including three [cellulose synthase (CSLD2), SHAVEN3-like 1 (SVL1), SMALL AUXIN UP RNA (SAUR21)] that are highly related to root development in other crops, were identified that might play important roles in AR formation in tea cuttings.NAA promotes the formation of AR of tea cuttings in coordination with endogenous hormones. The most important endogenous AR inductor, IAA, was reduced in response to NAA. DEGs potentially involved in NAA-mediated AR formation of tea plant stem cuttings were identified via comparative transcriptome analysis. Several hub genes, such as CSLD2, SVL1 and SAUR21, were identified that might play important roles in AR formation in tea cuttings.

4 citations

Journal ArticleDOI
01 Jul 2004
TL;DR: In this article, it was shown that the acceleration of Japanese pear flower development is accompanied by notable changes in endogenous hormone levels in the lateral buds, and it was suggested that depletion of the level and/or the activity of auxin in shoot tissues is responsible for the increase of bioactive cytokinin concentration in lateral buds.
Abstract: Shoot bending (30-45°) is known to accelerate flower development in Japanese pear, and here it is shown that this acceleration of flower development is accompanied by notable changes in endogenous hormone levels in the lateral buds. Indole-3-acetic acid (IAA) concentration in the lateral buds on vertical shoots increased in mid-July, but that in the bent shoot remained nearly constant. On the other hand, the concentrations of zeatin-type cytokinins in buds were higher in bent shoots than vertical shoots. Additionally, when maleic hydrazide, an anti-auxin, was applied to the pear shoots at 2,600 mg L-1, zeatin, zeatinriboside, and isopentenyladenine levels in lateral buds were increased whereas isopentenyladenosine concentration was decreased. Thus, it is supposed the depletion of the level and/or the activity of auxin in shoot tissues is responsible for the increase of bioactive cytokinin concentration in lateral buds. It is proposed that these changes of endogenous hormones may be involved in the acceleration of the lateral bud development.

4 citations

Journal ArticleDOI
TL;DR: In this paper, the authors established a convenient media for plant regeneration and embryogenesis from nodal segments excised from greenhouse-grown plants and cultured on MS (200 mM NaCl-free/not) medium.
Abstract: Rosa chinensis Jacq. is used worldwide in ornamental and medicine. The goal of this study was to establish a convenient media for plant regeneration and embryogenesis from nodal segments excised from greenhouse-grown plants and cultured on MS (200 mM NaCl-free/not) medium. Salinity stress could cause embryo death, plantlet morphological change and drastic necrosis during rooting and pot soil transplantation. The most effective embryo development (Dv), proliferation (Pl) and differentiation (Df) media under salt stress were respectively supplemented with [2.00 mg/L zeatin, 0.30 mg/L α-napthleneacetic acid (NAA), 1.50 mg/L gibberellic acid (GA3)]; [5.00 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D), 0.50 mg/L zeatin, 1.50 mg/L GA3] and [2.00 mg/L zeatin, 1.50 mg/L GA3, 3.00 mg/L abscisic acid (ABA)]. These media provided an average of 100±0.00, 88.35±0.68 and 51.59±1.78% respectively as relatively high percentage of well developed embryos. Analysis of macromolecules nitrogen (N), phosphorus (P) and potassium (K) of 200 mM NaCl stress in vitro (SSIV) were significantly higher compare to control and 200 mM NaCl-free recovered in vitro (RIV) plantlets. Chlorophyll in contrary was significantly higher in control and RIV compare to SSIV plantlets. Interestingly, Water and proline remained significantly higher in RIV (371.42±0.88 µg/g and 69.24±0.65% respectively) compare to control (368.79±0.58 µg/g and 54.67±1.31% respectively) and thus, China rose was improved of physiological activity. Key words: Embryogenesis, plant regeneration, Rosa chinensis Jacq. ,transplantation, salinity stress.

4 citations

Journal ArticleDOI
TL;DR: In this paper, young Norway spruce seedlings were incubated under hydroculture conditions in a synthetic nutrient medium containing either 3H-isopentenyladenosine, isopenteniene, or zeatin riboside.
Abstract: Roots of young Norway spruce seedlings were incubated under hydroculture conditions in a synthetic nutrient medium containing either 3H-isopentenyladenosine, isopentenyladenosine or zeatin riboside. When feeding with 3H-isopentenyladenosine a new radiaolabelled metabolite was found in the feeding solution as well as in root extracts. Isopentenyladenosine and zeatin riboside were metabolised and for both compounds an unknown metabolite was detected in the feeding solution. The metabolites were purified by solid phase extraction, HPLC and partially characterised. A major characteristic of the metabolites is their reactivity in the presence of NH4OH, which results in the formation of the cytokinin bases isopentenyladenine or zeatin, respectively. UV-spectra and the chemical characteristics indicate that the new metabolites are closely related. The GC-MS analysis revealed, that the metabolites are true derivatives of isopentenyladenine and zeatin. The biogenesis of the new metabolites is discussed with regard to plant microbial interactions.

4 citations

Journal ArticleDOI
TL;DR: In this paper, the behavior of the naturally occurring cytokinin zeatin 1 in aqueous acidic conditions has been studied, after it was observed that this compound was relatively more stable than 6-(3-methylbut-2-enylamino)purine 2.
Abstract: The behaviour of the naturally occurring cytokinin zeatin 1 in aqueous acidic conditions has been studied, after it was observed that this compound was relatively more stable than another related cytokinin, 6-(3-methylbut-2-enylamino)purine 2. Zeatin 1 reacted readily in 1 mol dm–3 aqueous HCl at 100 °C. Three products were characterized, a diol resulting from the hydration of the double bond of the aliphatic chain, and two cyclized products, a hydroxypyrrolidine and a dihydropyrrole. A scheme is proposed to explain the formation of these products. However, zeatin was found to be stable in 0.1 mol dm–3 aqueous HCl at 100°C and in 1 mol dm–3 aqueous HCl at 50 °C, conditions which allow the hydrolysis of zeatin riboside.

4 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202333
2022103
202135
202034
201932
201848