Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Optimization of Growth Regulators for Shoot Induction and Regeneration of Tomato (Lycopersicum esculentum Mill.)

Abstract: 2 Abstract: Until now numbers of efforts have been made to deduce the optimized conditions for the in vitro regeneration of tomato plant. The optimized conditions deduced are contradictory to each other and to validate the optimum conditions the present investigation was carried out which directs the use of 1.5 mg/l IAA + 1.5 mg/l Zeatin with full strength Murashige and Skoog's (MS) medium for the development of callus, which was regenerated in to complete plants by shoot development at 1.5mg/l of Zeatin and 1.5 mg/l of IAA and rhizogenesis in the full strength MS medium supplemented with 1.5 mg/l IAA.

Endogenous Cytokinins in Bougainvillea 'San Diego Red.' II. The Complex in Mature Leaves

Abstract: Nine compounds that stimulated division of soybean callus cells could be tentatively identified in the mature leaves of Bougainvillea `San Diego Red.' These included phosphorylated and glucosylated forms of zeatin, zeatin riboside, and their dihydro derivatives. The cytokinin glucosides detected in mature leaves may not have been formed entirely in situ as a result of metabolism but may have originated partly from the roots.

Transcriptome analyses provide insights into the homeostatic regulation of axillary buds in upland cotton (G. hirsutum L.).

Abstract: The axillary bud is an important index of cotton plant-type traits, and the molecular mechanism of axillary bud development in upland cotton has not yet been reported. We obtained a mutant (designated mZ571) with a high-budding phenotype in axillary bud development from the low-budding phenotype variety G. hirsutum Z571 (CCRI 9A02), which provided ideal materials for the study of complex regulatory networks of axillary bud development. In this study, RNA sequencing was carried out to detect gene expression levels during three stages of axillary buds in Z571 (LB, low budding) and mZ571 mutant (HB, high budding). A total of 7162 DEGs were identified in the three groups (HB-E vs. LB-E, HB-G1 vs. LB-G1, HB-G2 vs. LB-G2), including 4014 downregulated and 3184 upregulated DEGs. Additionally, 221 DEGs were commonly identified in all three groups, accounting for approximately 3.09% of the total DEGs. These DEGs were identified, annotated and classified. A significant number of DEGs were related to hormone metabolism, hormone signal transduction, and starch and sucrose metabolism. In addition, 45, 22 and 9 DEGs involved in hormone metabolic pathways and 67, 22 and 19 DEGs involved in hormone signal transduction pathwayspathway were identified in HB-E vs. LB-E, HB-G1 vs. LB-G1, and HB-G2 vs. LB-G2, respectively, suggesting that endogenous hormones are the primary factors influencing cotton axillary bud growth. Hormone and soluble sugar content measurements revealed that mZ571 exhibited higher concentrations of zeatin, gibberellins and soluble sugar in all three stages, which confirmed that these hormone metabolism-, hormone signal transduction- and starch metabolism-related genes showed interaction effects contributing to the divergence of axillary bud growth between mZ571 and Z571. Our results confirmed the importance of endogenous hormones and sugars in the development of axillary buds, and we found that mZ571 plants, with a high-budding phenotype of axillary buds, exhibited higher endogenous hormone and sugar concentrations. Overall, we present a model for the emergence and development of cotton axillary buds that provides insights into the complexity and dynamic nature of the regulatory network during axillary bud emergence and development.

In vitro multiplication of lingonberry - Short Communication

Abstract: Paprstein F., Sedlak J. (2015): In vitro multiplication of lingonberry – Short Communication. Hort. Sci. (Prague), 42: 102–106. Although plants of Vaccinium genus have not been cultivated on a large scale in the Czech Republic, there is a potential for commercial lingonberry production in some mountain regions. The purpose of this study was to develop an efficient in vitro system for a quick multiplication of lingonberry cvs Koralle, Linnea, and Runo Bielawskie. McCown woody plant medium (WPM), Anderson’s rhododendron medium (AN) and half-strength Murashige and Skoog medium (half-MS) containing cytokinin zeatin in concentrations 0.5, 1 or 2 mg/l were tested for repeated subcultures. The number of newly formed shoots varied with the cultivar, medium tested and concentration of zeatin. Across all experiments, the highest multiplication rate (8.9 ± 0.6) was obtained for cv. Runo Bielawskie on WPM medium with the highest concentration 2 mg/l of zeatin. The lowest multiplication rates 1.1 ± 0.0 were noted on half-MS medium with the lowest concentration of zeatin (0.5 mg/l). In conclusion, micropropagation techniques described in this paper increased multiplication mainly in lingonberry cv. Runo Bielawskie on WPM medium. However, some cultivars of lingonberry would still require further research to optimize proliferation media.

Growth Promotion of Tomato Seedlings by Applicaion of Bacillus sp. Isolated from Rhizosphere

Abstract: Two bacterial strains isolated from soil (Bacillus subtilis strains: PS2 and RFO41) were evaluated to determine their promoting effect on the growth of tomato seedling under axonic and pot conditions. The production of phytohormone, such as indole-3-acetic acid, indole-3-butyric acid, gibberellin and zeatin by these two strains was investigated as possible mechanisms for plant growth stimulation. Both PS2 and RFO41 were shown to produce various phytohormones, and. the production of phytohormones was stimulated by the addition of peptone-rich brain heart broth medium. In addition, these bacteria exhibited high levels of phosphatase activity, which ranged from 2.18 to . PS2 and RFO41 were applied to the pot test for growth of tomato seed with phosphate. Root and shoot lengths of germinated tomato after 15 days were 45.5% and 36.5% longer than that of control in RFO41 treated samples, respectively. Baciller sp. PS2 and RFO41 may have a potential for biofertilizer in the agriculture.