Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Nutrient and hormone levels in cotton ovules during embryony

Abstract: In vitro zygotic and somatic embryogenesis protocols rely on nutrient and hormone levels from media to satisfy the physiological and developmental requirements of embryony. To better understand these requirements for cotton, we quantified levels of major and minor elements, carbohydrates, NH4 +, free amino acids and six hormones in whole cotton ovules (with fibers removed), nucelli (ovules with integuments removed), or ovule fluid (extracted from the endosperm region). Samples were collected from field-grown cotton at 1–18 days-past-anthesis (DPA) during each of three growing seasons. Replication across 2 years was obtained for carbohydrates, NH4 +, free amino acids and hormones from nucellus samples. The year effect was large primarily for hormones only. The most abundant minerals across tissue types and years were K, P, Mg and S. Potassium was the most abundant at 260, 600 and 1,660 mmol kg−1 dry mass (DM) in nucelli, whole ovules and ovule fluid, respectively. Magnesium, Ca, Zn and Mn levels were 2–8-fold higher in ovule fluid compared to whole ovules or nucelli. In the free amino acid plus NH4 + category, NH4 +, alanine, serine, glycine, asparagine (plus aspartic acid), glutamine (plus glutamic acid), leucine, threonine and arginine predominated in nucelli and ovule fluid, and levels tended to be higher in the older samples across years and tissue types. Fructose and glucose levels also increased with age with very high levels being found in late DPA ovule fluid. Arabinose, inositol and melibiose were also prominent sugars. Indole-3-acetic acid levels were similar between nucelli and ovule fluid and ranged from 10 to 80 μmol kg−1 DM. An abscisic acid spike, from 15 to 400 μmol kg−1 DM, occurred in nucelli and whole ovules from 2 to 8 DPA. Thereafter, abscisic acid levels remained between 5 and 10 μmol kg−1 DM. Zeatin and zeatin riboside were the most abundant cytokinins, and levels of these hormones fluctuated between 1 and 4 μmol kg−1 DM in both nucelli and ovule fluid.

Plant growth regulator and application method thereof

Abstract: The invention belongs to the technical field of pesticides, and particularly relates to a plant growth regulator and an application method thereof. Effective components of the plant growth regulator comprise alternaria tenuissima activated protein and any one selected from diethyl aminoethyl hexanoate, zeatin and compound sodium nitrophenolate. According to the plant growth regulator, and wettablepowder, water dispersible granule, suspension and water aqua thereof, the alternaria tenuissima activated protein and any one selected from diethyl aminoethyl hexanoate, zeatin and compound sodium nitrophenolate are mixed with each other to be used as the effective components, and the action mechanisms are different; after compounding, a synergistic effect is achieved under a defined usage concentration and usage method according to various crops such as rice, corns, wheat, peanuts, cotton, vegetables, tobaccos, fruit trees, gardens, flowers and the like, and the yield and the income are increased; an obvious weight-increasing effect is achieved for fruits of the fruit trees, and the chlorophyll content of plants is effectively improved; and moreover greenness preservation and an anti-aging effect are achieved, the sugar content is improved, growth of the crops is promoted, and quality of the fruits is effectively improved.

Micropropagation of Harpagophytum procumbens (Burch.) DC. ex Meisn.; the effect of cytokinins on shoot multiplication.

Abstract: The procedure of in vitro propagation of Harpagophytum procumbens using shoot tips was developed. Shoot tips were cultured on Schenk and Hildebrandt (SH) agar medium supplemented with 0.57 μM indole-3-acetic acid (IAA) in combination with various cytokinins: 6-benzylaminopurine (BAP), thidiazurone (TDZ), kinetin or zeatin at four concentrations (2, 4, 6 or 8 μM). The best shoot multiplication rate (11.2 shoots/explant for 5 weeks) was achieved in the presence of 6 μM TDZ. The shoots were small and their elongation on SH medium supplemented with gibberellic acid (GA3) was necessary. Shoots of H. procumbens were rooted on full-strength or half-strength Murashige and Skoog (MS) agar medium either with or without auxin. Plantlets were transferred into pots and maintained in the greenhouse. After 1 month, the overall survival of plants was 83% but it decreased to 27% after 6 months.