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Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.


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TL;DR: Spring was –for all varieties– the most favourable time to collect explants: the highest sprouting rates and the lowest contamination levels were obtained, Frantoio and Manzanilla being the varieties least affected by contamination.
Abstract: Plant sanitation through in vitro culture helps addressing sanitary issues that affect olive production (Olea europaea L.). Our goal was to develop a protocol for in vitro establishment of plant material obtained from adult plants grown in the field. We tested four methods of disinfection, the explants response to two cytokinins (BA, zeatin), two carbon sources (sucrose, mannitol), and activated charcoal (0 y 1 g.L-1), and the impact of the season in which materials were collected. The use of ultrasound during disinfection allowed satisfactory establishment rates (44 %). BA improved sprouting (36 % vs. 24 %), and so did mannitol (75 % vs. 20 %). The addition of activated charcoal did not show statistically significant effects on both survival and sprouting. We observed variations depending on the season of collection and the plant variety. Spring was –for all varieties– the most favourable time to collect explants: the highest sprouting rates (21 % to 62 %) and the lowest contamination levels were obtained, Frantoio and Manzanilla being the varieties least affected by contamination.

3 citations

Journal ArticleDOI
TL;DR: In this article , RNA sequencing technology combined with ultra-performance liquid chromatography and tandem mass spectrometry were used to analyze the changes in the leaf transcriptome and metabolomics of soybean leaves at the seedling stage under DPC stress.
Abstract: Soybeans are an important economic crop. As the most widely used growth regulator globally, the molecular mechanism of mepiquat chloride (DPC) in soybean remains unknown. In this study, RNA sequencing technology combined with ultra-performance liquid chromatography and tandem mass spectrometry were used to analyze the changes in the leaf transcriptome and metabolomics of soybean leaves at the seedling stage under DPC stress. The results showed that differentially expressed genes related to photosynthesis and cell wall synthesis were significantly downregulated at the transcriptional level. In addition, the syntheses of gibberellin, zeatin, brassinolide, and other plant hormones were inhibited in the signal transduction pathway of plant hormones, thereby inhibiting plant growth. In contrast, at the metabolic level, the expression levels of flavonoid differential metabolites were significantly increased, and the proportions of flavonoids in the two varieties were 61.5 and 66%, respectively. The combined analysis of transcriptome and metabolomics showed that the differential expressed genes and metabolites were mainly enriched in the isoflavonoid biosynthesis and flavonoid biosynthesis pathways. Principally, DPC inhibited plant growth but improved drought resistance. Our study is the first to report the molecular mechanism of DPC regulation in soybean, providing useful insights into the rational application of DPC in soybean.

3 citations

01 Jan 2006
TL;DR: This is the first report on synthetic seed production and retrieval of viable plantlets in Agave vera-cruz and obtained plantlets were directly transferred to pots containing sand:soil:manure in the ratio of 1:1:1 without hardening.
Abstract: In vitro shoot buds and somatic embryos from organogenic and embryogenic calli respectively derived from shoot tip cultures of Agave vera-cruz were isolated and encapsulated with sodium alginate and dihydrated calcium chloride. These alginate beads were placed on nutritive medium containing MS salts and L2 vitamins with 3 sucrose and stored in a domestic refrigerator at 5±2°C and freezer at - 5±2°C for 7, 14 and 21 days. After storage, synseeds were dehydrated, thawed and placed on recovery media, containing salts of Murashige and Skoog's medium and L2 vitamins supplemented with IBA (4.92I¼M) + BAP(8.86I¼M) + GA3(4.92I¼M) and NAA (5.37I¼M) + Zeatin (0.91I¼M) + 40gms of sucrose for encapsulated shoot buds and somatic embryos respectively. The conversion frequency of artificial seeds to seedlings varied from 40 to 53 after storage. Thus obtained plantlets were directly transferred to pots containing sand:soil:manure in the ratio of 1:1:1 without hardening. Nearly 75 to 80 of survival was recorded. This is the first report on synthetic seed production and retrieval of viable plantlets in Agave vera-cruz. © 2006 Society for Biology and Biotechnology.

3 citations

01 Jan 2014
TL;DR: An efficient and reproducible direct regeneration protocol has been developed in cultivated tomato cv Samrat-18 using cotyledon explants which is genotype dependent.
Abstract: An efficient protocol for multiple shoots induction and plant regeneration from cotyledon explants of cultivated tomato (Lycopersicum esculentum Mill.) cv Samrat-18 is reported. The cotyledon explants excised from of 10-12 days old in vitro grown seedlings were cultured on MS medium supplemented with 0.50- 1.2 mg/L Zeatin as a sole growth regulator and also in combination with 0.1/0.5 mg/L IAA. Maximum number of multiple shoots formation was found at 1.0 mg/L Zeatin with 0.1 mg/L IAA. Shoots were transferred to MS medium supplemented with 0.2-1.0 mg/L GA3 for elongation. The medium supplemented with 0.6 mg/L GA3 in showed the maximum percentage of enhancement of shoot elongation. For In vitro rooting, elongated shoots were excised and transferred onto MS medium supplemented with 0.5mg / L IBA, IAA and NAA. Profuse rhizogenesis was observed at 0.5 mg/L IBA compared to IAA / NAA. The regenerated plants were acclimatized in the culture room and maintained in the green house. These plants were found to be normal and similar to the donor plant. Thus, an efficient and reproducible direct regeneration protocol has been developed in cultivated tomato cv Samrat-18 using cotyledon explants which is genotype dependent.

3 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202333
2022103
202135
202034
201932
201848