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Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.


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Journal ArticleDOI
01 Mar 2019-Vegetos
TL;DR: A successful regeneration system for an elite Indian Musa sp.
Abstract: An efficient and rapid regeneration system is indispensable for germplasm conservation and valuable trait incorporation through genetic engineering. The present study was aimed to investigate regeneration potential of Musa sp. cv. Somrani monthan (ABB) via somatic embryogenesis. Meristematic shoot tips were used as explant and cultured on MS medium supplemented with 6-BAP (50 and 100 µM) and IAA (1 µM) for scalp (cauliflower like compact buds) induction. The highest percentage (~ 60%) of homogenous differentiation of shoot tip explants into scalp was observed in MS medium supplemented with 100 µM of 6-BAP and 1 µM of IAA after 6 months of culture. The scalps were sub-cultured on MS medium supplemented with different compositions of 2, 4-D (1, 2, 3, 4, 5 and 10 µM) and zeatin (1 and 2 µM) for embryogenic calli induction. The highest frequency (97%) of embryogenic calli induction was recorded in MS medium supplemented with 5 µM 2, 4-D and 1 µM zeatin after 6 weeks of culture. The histological and morphological studies suggest that, callus possessed high competence for embryogenesis and could be induced to form somatic embryos. Embryogenic cell suspension (ECS) was successfully established by culturing these embryonic calli in liquid medium. About 70% of the mature somatic embryos germinated and converted to plantlets with a regeneration capacity of 8.5 × 103. After the gradual acclimatization and hardening, the plants were transferred to nursery with 82% of survival rate. Here we report a successful regeneration system for an elite Indian Musa cv. Somrani monthan via somatic embryogenesis. The embryogenic callus and ECS are ideal explants for mass clonal propagation, germplasm conservation and genetic transformation for future research.

3 citations

Journal ArticleDOI
TL;DR: Nine functional modules comprising of different hub genes which contribute to the cytokinin signaling route are identified and will be useful to design new stress-resistant cultivars which can provide sustainable yield in stress-specific conditions.
Abstract: Rice is an important staple food grain consumed by most of the population around the world. With climate and environmental changes, rice has undergone a tremendous stress state which has impacted crop production and productivity. Plant growth hormones are essential component that controls the overall outcome of the growth and development of the plant. Cytokinin is a hormone that plays an important role in plant immunity and defense systems. Trans-zeatin is an active form of cytokinin that can affect plant growth which is mediated by a multi-step two-component phosphorelay system that has different roles in various developmental stages. Systems biology is an approach for pathway analysis to trans-zeatin treated rice that could provide a deep understanding of different molecules associated with them. In this study, we have used a weighted gene co-expression network analysis method to identify the functional modules and hub genes involved in the cytokinin pathway. We have identified nine functional modules comprising of different hub genes which contribute to the cytokinin signaling route. The biological significance of these identified hub genes has been tested by applying well-proven statistical techniques to establish the association with the experimentally validated QTLs and annotated by the DAVID server. The establishment of key genes in different pathways has been confirmed. These results will be useful to design new stress-resistant cultivars which can provide sustainable yield in stress-specific conditions.

3 citations

Journal ArticleDOI
TL;DR: Shoot regeneration has been achieved in Zizyphus mauritiana from juvenile explants (internodal and nodal segments) on MS medium containing 2% sucrose and 50 mg l−1 each of asparagine, arginine and glutamine.
Abstract: Shoot regeneration has been achieved in Zizyphus mauritiana from juvenile explants (internodal and nodal segments) on MS medium containing 2% sucrose and 50 mg l−1 each of asparagine, arginine and glutamine, 5 mg l−1 cystelne hydrochloride and various combinations of IAA/zeatin, BAP, KN and Ad. The explants were most responsive on the medium containing zeatin followed by BAP. Callusing could be induced from all parts of the seedling viz. cotyledonary leaf, hypocotyl, epicotyl, leaf and nodal region on various media tried. Expression of peroxidase and esterase in the in vivo and in vitro grown tissues was also compared through starch gel electrophoresis.

3 citations

Journal Article
TL;DR: This study carried out to investigate the optimal condition for multiple propagation through leaf tissue culture and to apply anther culture techniques to Pulsatilla koreana Nakai breeding, finding the best combination for plant regeneration in callus induced from anther was 1650mg/l + 3800mg/L + zeatin 2.0mg /L.
Abstract: This study was carried out to investigate the optimal condition for multiple propagation through leaf tissue culture and to apply anther culture techniques to Pulsatilla koreana Nakai breeding. Leaf and anther of Pulsatilla koreana Nakai were cultured on MS, MT, LS and media supplemented with several growth regulators and nitrogen sources under various conditions. For callus induction and differentiation from the Pulsatilla koreana leaf segments were more effective in the combination of zeatin and auxin than auxin alone. The color of the callus was green when treated with IBA alone. Shoot differentiation was more effective when treated with zeatin than auxin alone, especially the best hormoal combination for shoot differentiation was zeatin 1.0mg/l +NAA 0.1mg/l, while 2,4-D inhibited shoot differentiation. The appeared rate of S pollen was 35% in vivo, while that of S pollen by low temperature pretreatment for 4 days was increased by 53% and the optimum culture time for callus induction from anther was uni-nucleate stage. basal medium supplemented with NAA 0.5mg/l and zeatin 1 mg/l was the most effective on callus formation and the best results of plant regeneration were obtained from combination of NAA 0.5mg/l and zeatin 0.5mg/l in anther culture. as more effectives as the nitrogen source than and the combination with zeatin 2.0mg /L was the best effective. The best combination for plant regeneration in callus induced from anther was 1650mg/l + 3800mg/l + zeatin 2.0mg/l. Ploidy level of anther-derived plants appeared 28% haploid, 47% diploid and the others were triploid, tetraploid and mixploid. In compare with E.S.T, M.D.H and P.X banding patterns were distinguished among callus, haploid and diploid plants in electrophoresis.

3 citations

Journal ArticleDOI
TL;DR: A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint and the plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.
Abstract: A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint (Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L(-1) zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L(-1)) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half- strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96% survival.

3 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202333
2022103
202135
202034
201932
201848