Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Production of tissue culture of plant

Abstract: PURPOSE:To enable production of a tissue culture from rhizomatic cells of Ficus benjamina L and petiolar cells of Ficus retusa L by bedding protoplasts of somatic cells of an arboreous ornamental foliage plant in a culture solution containing a plant growth hormone and culturing the protoplasts CONSTITUTION:Protoplasts of an arboreous ornamental foliage plant, such as Ficus benjamina L or Ficus retusa L, belonging to the genus Ficus of the family Moraceae are bedded in a culture solution prepared by adding a plant growth hormone consisting of auxin-based dichlorophenoxyacetic acid or 1- naphthaleneacetic acid alone in <=1ppm concentration and cytokinin-based zeatin alone in <=1ppm concentration added to a culture medium containing inorganic salts and vitamins as a base and cultured at about 20 degC atmospheric temperature in the dark to primarily divide cells for a culture period of 30 days without inactivating the protoplasts Thereby, a tissue culture, such as colony or callus, is obtained As a result, a cloned plant, such as Ficus retusa L or Ficus benjamina L, can be regenerated The protoplasts of the ornamental foliage plant can be cultured without utilizing nurse culture

Effect of coconut water, darkness and auxins on morphogenesis of Ariocarpus kotschoubeyanus (Cactaceae)

Abstract: This study describes a method to micropropagate Ariocarpus kotschoubeyanus, using coconut water and darkness conditions. The longitudinal explants from seedlings germinated in vitro were cultivated on Murashige and Skoog medium (MS) with 2 mg/l zeatin. The calli generated were transferred to medium containing 5 ml, 10 ml or 15 ml of coconut water per litre. After 84 days, five green shoots per callus were generated under photoperiod but 14 well defined shoots were produced in darkness. The shoots were cultivated on media with activated charcoal and polyethyleneglycol to reduce callus and hyperhydricity, respectively. To induce rooting the effect of the auxins indolacetic acid and indolbutyric acid (1 mg/l) were tested. The roots type II (20 to 30 mm) and type III ( > 30 mm) were observed after 120 days onto media containing auxins but not in control medium. The regenerated plants were transferred to horticultural earth, peat moss and sand (30:30:40) and the survival of the plants in soil was 80%. The plant adaptation was complete and the first flowering was observed after 1 year in greenhouse conditions. The procedure developed is an alternative to the successful propagation of A. kotschoubeyanus and reduces the risk of extinction of this species.