scispace - formally typeset
Search or ask a question
Topic

Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.


Papers
More filters
Patent
30 Sep 1992
TL;DR: In this article, a new kind of plant growth regulator-lodging-resistant panacea, is introduced, which is a woter preparation of ethephon compounded with one or several kinds of division element of cytokinin.
Abstract: A new kind of plant growth regulator-lodging-resistant panacea, is a woter preparation of ethephon compounded with one or several kinds of division element of cytokinin, 6-benzylaminopurine, 6-furfuryl-aminopurine, zeatin, etc.. This preparation can raise the lodging-resistant ability of grain crops, and also has the obvious effect of increasing the output under the condition of no lodging of the crop.

3 citations

Journal ArticleDOI
TL;DR: The HPLC analysis recommended that TDZ is more effective in accumulation of individual phenolic and flavonoid than Zeatin and provided a useful system for further study on in vitro culture of G. jasminoides as alternative and new source for important secondary products.
Abstract: BACKGROUND AND OBJECTIVE Gardenia (Gardenia jasminoides) has many pharmacological actions such as anti-inflammatory, antioxidant and fibrolytic activities and cytotoxic effects, etc. This study was conducted to recognize the effect of zeatin and thidiazuron (TDZ) on callus proliferation, total phenolic content, total flavonoids and DPPH scavenging activity of gardenia callus cultures. MATERIALS AND METHODS Calli were cultured on Murashige and Skoog (MS) medium supplement with different concentrations (2, 4 or 6 mg L-1) of zeatin or TDZ individually as well as combination of 2 mg L-1 zeatin+4 mg L-1 TDZ. Cultures contained 4 mg L-1 TDZ gave the highest callus fresh weight followed by those contained 2 mg L-1 zeatin then that cultured on 4 mg L-1 zeatin. Data reported as Mean±Standard Deviation (SD). Data were subjected to one-way analysis of variance (p< 0.05). Results were processed by Excel (2010) and SPSS Version 17.0. RESULTS It was found that callus growing on medium supplemented with 4 mg L-1 zeatin gave the maximum value (14.93%) of yield extract. Callus cultured on 4 mg L-1 zeatin recorded the maximum total phenol (268.33 mg GAE/100 g FW of callus) and total flavonoids (2703.33 μg QE/100 g FW of callus) accumulation. The antioxidant activity of each extract was determined through DPPH radical scavenging activity. Callus cultured on 4 mg L-1 TDZ showed the highest antioxidant activity then those cultured on 4 mg L-1 zeatin. The HPLC analysis for phenolic acids showed that chlorogenic acid, rosmarinic acid and cinnamic reached their highest contents with callus cultured on 4 mg L-1 TDZ (123.24, 322.14 and 278.22 μg g-1, respectively). Regarding flavonoids and using HPLC analysis, rutin, apigenin-7-glucoside and kaempferol were detected. Callus cultured with 4 mg L-1 TDZ gave the highest rutin and kaempferol contents (287.76 and 10.38 μg g-1, respectively). However, apigenin-7-glucoside was detected with high content (129.86 μg g-1) in callus culture with 4 mg L-1 Zeatin. CONCLUSION The HPLC analysis recommended that TDZ is more effective in accumulation of individual phenolic and flavonoid than Zeatin. The present study provided a useful system for further study on in vitro culture of G. jasminoides as alternative and new source for important secondary products.

3 citations

Journal ArticleDOI
TL;DR: In this paper, the cytokinin type, the light quality and the explant orientation in the culture medium that favor the in vitro multiplication of blueberry shoots cv.Delite was determined.
Abstract: The objective of this work was to determine the cytokinin type, the light quality and the explant orientation in the culture medium that favor the in vitro multiplication of blueberry shoots cv. Delite. In the first experiment, the treatments applied were two cytokinins in the culture medium (2iP [25 M] and zeatin [18 µM]) and two light qualities (white and green). In the second experiment, the treatments were constituted of two cytokinins used in the culture medium (2iP [25 µM] and zeatin [18 µM]) and two explants orientations in the culture medium (vertical and horizontal). In both experiments the completely randomized experimental design was used, in factorial outline 2 x 2. The basal culture medium, used in the two experiments, was constituted by the WPM salts and the MS vitamins, added of myo-inositol (100 mg.L-1), sucrose (30 g.L-1) and agar (6 g.L-1). To the 30 days in culture it were evaluated the mean number of shoots and buds for explant, the mean length of the shoots and the length of the shoot more developed (major). In the second experiment it was also determined the multiplication rate. It was observed that the in vitro multiplication of blueberry shoots cv. Delite is favored with the cultivation of the explants in culture medium with zeatin (18 µM) and under white light. The explants cultivated in the horizontal orientation in the culture medium had larger mean number of shoots.

3 citations

Dissertation
01 Jan 2001
TL;DR: In this paper, different cytokinins, singly or in combination with auxins (IBA and NAA), were tested for shoot proliferation activity, and the optimal combinations were kinetin/NAA (2.0/0.1 mgl-1), zeatinllBA (1.5/1.0 mgl − 1), BA/IBA ( 1.0 /1.
Abstract: Shoot cultures of Aloe polyphylla were initiated from young shoot explants of in vitro grown plants. The basal medium was MS medium (MURASHIGE and SKOOG, 1962), supplemented with 100 mgl-1myo-inositol, and 30 gl-1 sucrose. Agar (0.8 %) was used as the gelling agent. Different cytokinins, singly or in combination with auxins (IBA and NAA), were tested for shoot proliferation activity. All the cytokinins tested (kinetin, zeatin, iP, and BA) gave a good shoot proliferation response. The optimal concentrations for shoot proliferation of each of the cytokinins tested were: zeatin (0.5 mgl-1), kinetin (1.5 mgl-1), iP (1.0 mgl-1) and BA (1.5 mgl-1). In combination with auxins, the optimal combinations were kinetin/NAA (2.0/0.1 mgl-1), kinetinllBA (1.5/1.0 mgl-1), zeatinllBA (1.0/0.5 mgl-1), zeatin/NAA (1.0/1.0 mgl-1), BA/IBA (1.0/1.0 mgl-1), BA/NAA (1.5/0.1 mgl-1). Although it gave the highest number of shoots per explant, BA was responsible for hyperhydricity. Temperature and sucrose also influenced shoot proliferation. The optimal temperature was 25°C, while 30 gl-1 was the optimal concentration of sucrose for shoot proliferation. Plants rooted well in plant growth regulator-free MS medium. Amongst the potting mixtures tested, soil: sand: vermiculite (1:1:1 v/v) was the best with 98 % plantlet survival. In the second part of this project, Platycerium bifurcatum cultures were established using leaf explants. The basal medium was MS medium (MURASHIGE and SKOOG, 1962), supplemented with 100 mgl-1 myo-inositol and 30 gl-1 sucrose. For bud initiation, 1.0 mgl-1BA was used, while 0.8 % agar was used as the gelling agent. Three different strengths of MS medium (full, half, and one-quarter strength) without plant growth regulators were tested for further bud growth and development. Half-strength MS proved to be the best for further

3 citations

Journal Article
TL;DR: The time required from inoculation of explants with Agrobacterium to transfer of transgenic tomato plants to soil was only 70 days as compared to 3 to 4 months in standard tomato transformation protocols.
Abstract: Factors that influence transformation efficiency such as age of explants, plant growth regulator concentration, antibiotic concentration, Agrobacterium density, and infection time were optimized with a view to establish a high-throughput transformation protocol for tomato (Solanum lycopersicum L.) cv. PKM-1. Seedling explants were transformed using a binary vector carrying nptII gene, conferring kanamycin resistance by Agrobacterium mediated transformation. Transformation efficiency of 27 % and 17 % respectively was observed when cotyledon and hypocotyl explants were collected from 7 and 9 day old seedlings, co-cultivated with an Agrobacterium suspension of O.D600 of 0.4 and 0.2 for 30 and 10 minutes infection time respectively. Better shoot development was observed when shoots induced on cotyledonary and hypocotyl explants were cultured on modified MS medium containing 0.5 and 1 mg/L zeatin respectively. Supplementation of medium with 200 mg/L timentin effectively suppressed Agrobacterium overgrowth. Furthermore, the time required from inoculation of explants with Agrobacterium to transfer of transgenic tomato plants to soil was only 70 days as compared to 3 to 4 months in standard tomato transformation protocols.

3 citations


Network Information
Related Topics (5)
Abscisic acid
12.8K papers, 587K citations
88% related
Shoot
32.1K papers, 693.3K citations
86% related
Arabidopsis thaliana
19.1K papers, 1M citations
84% related
Photosynthesis
19.7K papers, 895.1K citations
83% related
Arabidopsis
30.9K papers, 2.1M citations
82% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202333
2022103
202135
202034
201932
201848