Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Phenolic Constituents of Vaccinium Species from Both Natural Resources and Micropropagated Plantlets

Abstract: Fruits and leaves of Vaccinium species have rich bioactive phytochemicals. These bioactive phytochemicals make these plants particularly valuable for the medical and food industry. This novel approach was designed to determine the phenolic content of Vaccinium species obtained from both micropropagated and naturally growing leaves. An efficient micropropagation protocol was developed to produce tree Vaccinium species plantlets via direct organogenesis. Lateral buds containing one or two leaves were cultured in McCown woody plant medium (WPM), supplemented with zeatin/indole-3-butyric acid (IBA) (1.0/0.1 mg L –1 ). In conclusion, Protocatechuic acid, Chlorogenic acid, Syringic acid and Routine phenolic compounds were determined in significant amounts. It has been determined that the phenolic compounds of leaves produced in tissue cultures is higher than the phenolic compounds obtained from naturally growing leaves.

Micropropagation of Cold Resistant Blackberry Cultivar 'Gazda'

Abstract: Blackberries are some of the most consumed berries in the world Areas planted with blackberry in Europe and especially in Romania are limited mainly due to low frost resistance of traditional varieties One of the most frost resist ant cultivar, suitable for cold Romanian winters, i s the variety 'Gazda' Due to several drawbacks of the co nventional propagation of this cultivar when using root cuttings, the efficiency of a micropropagation protocol was tested, in order to obtain high quali ty planting material For the in vitro culture establishment axillary dormant buds were i ncubated on MS medium supplemented with BAP Proliferation of the regenerated shoots was tested on MS and DKW media using different concentrations of plant growt h regulators: BAP, 4-CPPU and Zeatin BAP caused average proliferation rates but also generat ed large masses of basal callus 4-CPPU was the solely plant hormone that did not generate severe c allus at any concentration and also provided the highest proliferation rate It was also tested the callus induction potential from in vitro obtained leaves using 15 hormonal variants (5 hormone types in diff erent concentrations) For most variants it was achieved a substantial mass of callus, however no d irect regeneration was obtained In vitro plantlets were successfully rooted and acclimatized directly ex vitro in Jiffy-7 pellets

In vitro development and germination of immature olive embryos

Abstract: SUMMARY In vitro culture methods were used to germinate olive embryos prior to maturation. Fruit, seed and embryo development were established with consecutive sampling from 20 to 100 days after bloom. For that same period, embryo development and germination success were determined by in vitro culture trials using one-third strength MS medium with or without the addition of zeatin. For early developmental stages, when isolation of the embryo was difficult, a cut portion of the seed containing the embryo was used for culture. The embryos cultured within the cut seed portions germinated and formed normal plantlets. Histological observations indicated a close similarity between the natural and in vitro immature embryo differentiation pattern, progressing through preglobular, globular, heartshaped and torpedo-shaped stages. In some cases, however, the in vitro immature embryos developed or germinated abnormally. The presence of zeatin (0.25 mg 1-1) in the culture medium and the use of a cut seed-portion containing the immature embryo allowed in vitro germination sooner after bloom than previously obtained. On the contrary, zeatin was a handicap for mature olive embryo in vitro germination, which reached 100% seedling formation when no plant growth regulators were used.

Changes in essential oil composition, phenylalanine ammonia lyase gene expression and rosmarinic acid content during shoot organogenesis in cytokinin-treated Satureja spicigera (C. Koch) boiss. shoots

Abstract: This study investigated the effects of different concentrations of zeatin and thidiazuron on the accumulation of phenolics and activity of phenylalanine ammonia lyase gene in the micropropagated Satureja spicigera shoots. S. spicigera clones were generated from cultured nodal segments on Murashige and Skoog including vitamins medium supported with zeatin and thidiazuron concentrations. Shoots were subcultured three times in the same medium and harvested at the end of the third month. The expression levels of phenylalanine ammonia lyase gene were conducted using semi-quantitative reverse transcription polymerase chain reaction technique. Rosmarinic acid contents were determined using reverse phase-high performance liquid chromatography with a diode array detector. Genetic stability of regenerants was performed using random amplified polymorphic DNA markers. Essential oils from shoots were identified by gas chromatography–mass spectrophotometry analysis. It has been detected that 0.1 mg/L thidiazuron is more effective on the node formation and shoot elongation. Contrarily, 4.0 mg/L thidiazuron caused a significant increase in shoot multiplication. The highest biomass accumulation was calculated from medium including 1.0 mg/L zeatin. According to random amplified polymorphic DNA analysis, all banding profiles from micropropagated plants were monomorphic. Gas chromatography–mass spectrophotometry analysis showed that rising concentrations of zeatin and thidiazuron increased the thymol and decreased the carvacrol content. It was also found that rosmarinic acid production increased significantly at 1.0 mg/L zeatin application. However, thidiazuron applications had no positive effect on rosmarinic acid accumulation. Comparing phenylalanine ammonia lyase gene expression and rosmarinic acid accumulation in in vitro shoots, it was found that they were correlated.