Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Molecular profiling of the Mahanarva spectabilis salivary glands and phytohormonal response of elephant grass

Abstract: Signal perception in plants is determinant to trigger specific physiological changes that confer resistance during the early stages of the insect attack. Thus, molecular characterization of both insect effectors and plant hormonal regulators are essential in developing forage grasses resistant to infestation by spittlebug Mahanarva spectabilis. We evaluated the presence of effectors in the salivary glands of M. spectabilis that could enable the infestation of elephant grass (Pennisetum purpureum). The presence of an NTPDase-like enzyme was confirmed, and higher activity was observed in females that hydrolyzed ADP. High levels of long chain fatty acids, such as octadecanoid acid (19.5%), prostaglandin A2 (4.7%), eicosanoid acid and the phytohormone salicylic acid (~4.0 μg/g) were also detected in the salivary glands. After 24 h of infestation, the phytohormone profiles were altered in leaves damaged by M. spectabilis, suggesting that the insect effectors modulate the plant response. Our results suggest that the plant response modulation may be the result of crosstalk between the salicylic acid, jasmonic acid, abscisic acid and zeatin pathways, induced by molecules from the salivary glands. This shows that the spittlebugs can interfere with jasmonic acid and zeatin accumulation in elephant grass plants.

The aspects concerning in vitro tuberring at the potatoe varieties.

Abstract: The study comprises a protocol concerning the in vitro neoformation of potatoe minitubers, starting from the apical tissue (apex) detached from the sprouts obtained by forcing the mature potatoe tubers (Solanum tuberosum L). A number of varieties have been experimented: Désirée, Ostara, Eba, Cristian, Muresan, Super and Caty. The percentage of in vitro rooting at some of these varieties has been of about 45%, after two months and of over 85% after three months from the culture on mediums with phitohormones. The results depend on the variety and on the nature of phitohormones. The presence of the zeatin and of the benziladenine has proved efficient, and the dose of 0.5 mg/l AIB favourable for the rooting at all varieties, stimulating the diameter of the minitubers formed in vitro. On the witness variants (Vo,V1) the rooting is sporadic reaching at some explants at only 10%.

Purines, pyrimidines, and imidazoles. Part XXXI. Some reactions of the allylic group in the plant cell-division promoting factor, zeatin, and its nucleoside and nucleotide derivatives

Abstract: Attempted diphosphorylation of 6-(4-hydroxy-3-methylbut-trans-2-enylamino)-9-(2′,3′-O-isopropylidene-β-D-ribofuranosyl) purine with 2-cyanoethyl phosphate and dicyclohexylcarbodi-imide in pyridine followed by removal of protecting groups gave 6-(3-methyl-4-pyridiniobut-trans-2-enylamino)-9-β-D-ribofuranosylpurine 5′-phosphate, which was hydrolysed by an alkaline phosphatase to the riboside and by acid to the aglycone. The latter compound was synthesised from zeatin and methanesulphonyl chloride in pyridine, and also by reaction of 6-(4-chloro-3-methylbut-trans-2-enylamino)purine, (prepared from zeatin and methanesulphonyl chloride in NN-dimethylformamide) or, less readily, O-p-tolylsulphonylzeatin (from zeatin and toluene-p-sulphonyl chloride) and pyridine. Structures assigned to the compounds were confirmed by u.v. absorption, 1H n.m.r., and mass spectroscopic studies. The related 3-carbamoyl-1-[2-methyl-4-(purin-6-ylamino)but-trans-2-enyl]pyridinium chloride was obtained in a similar manner and the quaternisation of pyridine by allyl alcohols including zeatin in the presence of 2-cyanoethyl phosphate and dicyclohexylcarbodi-imide was shown to be general.

Separation, optimization, and quantification of cytokinins by a recently developed amide-embedded stationary phase

Abstract: In this study, some plant growth regulators known as cytokinins [kinetin (K), zeatin (Z), thidiazuron (TDZ), benzylaminopurine (BAP), and dimethylallylaminopurine (AAP)] were separated by HPLC using an amide-embedded mixed-mode stationary phase which was synthesized by Aral et al. in recent years. The effect of mobile phase content, mobile phase pH, buffer concentration, and temperature on separation process was studied. In addition, a quantitative determination of cytokinins from Salvia limbata extract was studied, and some validation parameters such as limit of detection (LOD), limit of quantification (LOQ), and relative standard deviation (RSD) were calculated as a range of 0.03–0.1, 0.1–0.26 mg/L, and 0.03–0.08, respectively.