Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Study of Genetic Fidelity of Somatic Embryos - Derived Plants of Gentiana kurroo Royle from Western Himalayas - Plant of Great Medicinal Value

Abstract: Aim: To study the effect of plant growth regulators on direct and indirect somatic embryogenesis on Gentiana kurroo Royle – an endangered medicinal plant. Place and Duration of Study: Department of Biotechnology, Dr Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan-173230, Himachal Pradesh, India, 2010-2013. Methodology: For induction of direct and indirect somatic embryogenesis petiole, leaf and petiole derived callus (after incubation in activated charcoal) were cultured on MS medium supplemented with a range of combinations of auxins (2,4-D, picloram, dicamba) and cytokinins (zeatin, kinetin). Results: Somatic embryos were observed from both direct and indirect method. The best performance was observed on MS basal medium supplemented 1.0mg/l dicamba. Development of somatic embryos was observed on MS basal+0.50mg/l GA3+1.0 mg/lKn and the maximum plantlets formation was achieved when somatic embryos (directly and indirectly induced) were shifted to half strength MS basal medium. Original Research Article British Biotechnology Journal, 4(5): 589-611, 2014 590 Conclusion: In direct pathway somatic embryos were in contact with maternal tissue in a suspensor like structure. In indirect pathway, the explants first proliferated to give rise to callus before embryoids were induced. Somatic embryos have their own vascular tissues, and can develop new plantlets independently.

Method for building regenerative system of kostelezkya virginica

Abstract: The invention relates to a method for buildsing-up a regeneration system for beach mallow, using beach mallow mature embryos as explants, comprising following steps: (1)explants surface sterilization, (2)tissue culture, with MS basal culture medium containing 0.5-2.0mg/L of 2,4-dichlorophe noxyacetic acid,(3)tissue subculture, with MS basal culture medium containing 0.3mg/L of heteroauxin and 0.3mg/L of zeatin, (4)adventitious bud culture, with MS or 1/2 MS basal culture medium containing 0.1-0.2mg/L of heteroauxin and 0.3-0.5mg/L of zeatin, (5) rooting culture, with MS basal culture medium free of hormones, (6)obtusifolia seedlings transplanting and hardening. The method is provided with a tissue culture rate, a adventitious bud culture rate, a rooting rate up to 86.8%,64.2% and 95.2% respectively, and the survival rate of regenerating seedlings up to 80%.

Novel callus inducing cell and production thereof

Abstract: PURPOSE:To collect a callus inducing cell capable of producing a saponin, by transplanting a callus cell induced from a tissue piece of a plant of the genus Bupleurum to a medium containing a plant growth regulator such as indoleacetic acid and cultivating. CONSTITUTION:A callus cell induced from a tissue piece of a plant of the genus Bupleurum is transplanted in a medium containing at least one plant growth regulator selected from indoleacetic acid, alpha-naphthaleneacetic acid and indolebutyric acid or at least one plant growth regulator such as indoleacetic acid, alpha-naphthaleneacetic acid and indolebutyric acid and at least one plant growth regulator selected from kinen, zeatin, dihydrozeatin, benzyladenine and isobenzyladenine and cultivated. A callus inducing cell is obtained from the culture mixture.

Effects of endogenous phenolic compounds and hormones on in vitro shoot tip culture of grapevine

Abstract: SummaryWe have examined changes in endogenous hormones [indole-acetic acid (IAA), gibberellic acid (GA3), zeatin (Z) and abscisic acid (ABA)], total phenolic contents and seven major phenolic compo...

A high trans-zeatin nucleoside concentration in corms may promote the multileaf growth of Amorphophallus muelleri

Abstract: Amorphophallus muelleri has a multileaf growth pattern different from that of other konjacs; however, the hormonal mechanism underlying this phenomenon is not clear. In this study, the levels of hormones closely related to the sprouting of the axillary bud, including five types of cytokinins, indole-3-acetic acid (IAA) and abscisic acid (ABA) were measured. In the second leaf sprouting stage, the content of trans-zeatin riboside (tZR) in corms increased more than 5000-fold over that in the dormancy period. Surprisingly, although the expression of CYP735A1 and CYP735A2, which synthesize the precursors for tZR was elevated at the second leaf sprouting stage, the expression of IPTs, which have key roles in cytokinin biosynthesis, did not change significantly. In addition, most cytokinin contents in leaves during the same period were significantly lower than those in corms. We speculate that the high cytokinin contents in the corms may not biosynthesized de novo in corms. In addition, the IAA content in the corms also considerably increased during the second leaf sprouting stage. Indole-3-acetaldehyde oxidase (AO1) and auxin efflux carrier PIN1A, presented relatively high expression levels in the same period. In contrast, ABA content, and the expression of NCED1, a rate-limiting enzyme in ABA biosynthesis, were suppressed at the second leaf sprouting stage. It is worth mentioning that N6-(Δ2-isopentenyl) adenosine (iP)-type cytokinins have a high content in corms in the dormant period that significantly decreases after the first leaf sprouting stage, which is completely different from the trend of tZR. By treating dormant corms with iP, the percentage of multibud plants increased, and the growth performance in terms of bud and root length was significantly higher than those of the control. This implies that iP-type cytokinins tend to play a role in promoting first seedling sprouting. Furthermore, there was a remarkable increase of the IAA content in both corms and roots under iP treatment but an inhibitory effect in buds. We speculate that the increase in the IAA content induced by iP is tissue specific. These results will assist in the understanding of the role of hormones, especially cytokinins, in the multileaf growth type of konjac.