Topic
Zeatin
About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.
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TL;DR: Powdered sulfur was used for the conversion of the protected ribosyl zeatin phosphonate derivatives 2 and 3 into the corresponding thiophosphate derivatives 8 and 9, whereas 2,2-dipyridyldisulfide in the presence of methanol was used to convert the 2,3,6,7,8 and 9 derivatives into corresponding methyl phosphates 6 and 7 as mentioned in this paper.
2 citations
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01 Jan 1999TL;DR: A new immunocytochemical method focusing on the localization of cytokinin bases which were evidenced to be the active forms is developed.
Abstract: Fundamental to expected responses when excised plant tissues are placed in vitro is the question to which extent expiant factors, especially the endogenous hormonal situation, are influencing the receptivity to external stimuli (Cassells et al., 1982). In addition, the main consideration of the recent approaches dealing with the regulation of gene expression by hormones has been the effect of exogenous compounds providing no information on the patterns of distribution of endogenous hormones. This is particularly true for cytokinins which are known to trigger a variety of developmental events both in whole plants and in in vitro cultures (Kaminek, 1992; Brzobohaty et al, 1994). Analytical methods to quantify cytokinins have been strongly improved during the recent past years, however, the various approaches to localize cytokinins in situ have long been questionable due to their diffusible property. Pioneer works in the area of cytokinin immunocytochemistry (Zavala, Brandon, 1983; Eberle et al., 1987; Sossountsov et al., 1988) stressed the role of aldehyde fixation in the linkage between the cytokinin bases isopentenyladenin (iP), zeatin (Z) and dihydrozeatin (DHZ) and BSA and also emphasized the use of antibodies obtained by immunization with cytokinin ribosides coupled to BSA according to the periodate oxidation method (Erlanger, Beiser, 1964). Following these works, we have developed a new immunocytochemical method focusing on the localization of cytokinin bases which were evidenced to be the active forms (Laloue, Pethe, 1982; Vesely et al., 1994).
2 citations
01 Jan 2014
TL;DR: Investigation of shoot proliferation of two genotypes of J. regia L. on three culture media containing different concentrations of diverse cytokinins showed that DKW medium was the best medium for the most of investigated parameters for walnut micropropagation.
Abstract: Walnut (Juglans regia L.) is one of the important nut trees in Iran and has a wide distribution and diversity in this country. Walnut propagation especially micropropagation has some problems. This study was carried out in order to investigate shoot proliferation of two genotypes (Z60 and Z63) of J. regia L. on three culture media (MS, WPM and DKW) containing different concentrations of diverse cytokinins. Cytokinins were BA, TDZ, Zeatin, Kinetin, at 0.0, 0.2, 0.4, 0.6, 0.8, mgl -1 combined with 0.01 mgl -1 IBA. Results showed that DKW medium was the best medium for the most of investigated parameters for walnut micropropagation. The highest number of shoots with callus (%) obtained in 0.8 mgl -1 TDZ. Media containing 0.8 mgl -1 BA produced the highest number of multiple shoots/explant and the highest number of leaves. Also, the longest shoot length (cm) obtained from media containing 0.8 mgl -1 Zeatin and also the highest numbers of nodes obtained from media containing Zeatin. High concentrations of BA produced explants with high shoot fresh weights and high concentrations of TDZ lead to produce explants with high callus fresh weights.
2 citations
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2 citations
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TL;DR: It would appear as if the cytokinin-like activity in geophyte leaves is rapidly utilized and/or metabolized during the resumption of vegetative growth and with the onset of flowering.
2 citations