Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Comparative assessment of The Effect of Moringa oleifera Leaf Extract (MLE) and Zeatin on invitro Regeneration Response of Pogostemon cablin Bud Explants

Abstract: Patchouli is one of the essential oil-producing plants which is commonly found in Indonesia, but the productivity tends to decline. Tissue culture technology is one alternative to increase patchouli plant productivity. Tissue culture requires growth regulators in order for the plants to grow optimally. Moringa plants have the potential as a source of growth regulators for tissue culture because Moringa leaves contain the zeatin hormone. Therefore, a comparison test of various concentrations of the zeatin and the moringa leaf extract is needed to determine whether the Moringa leaf extract can be an alternative to the zeatin hormone. This study aimed to investigate the effect of Moringa leaf extract on the growth of patchouli bud explants. The study employed a complete random design (CRD) with the treatment of concentrations of Moringa leaf extract in MS media. Moringa leaf extract was given at 30 grams/L, 40 grams/L, and 50 grams/L, respectively. Meanwhile, the zeatin hormone was given at a concentration of 1.5 ppm, 2 ppm, and 2.5 ppm. Each treatment was repeated four times. Moringa leaves were extracted by maceration method, and patchouli explants were obtained from the 3rd sub-culture of patchouli shoots. From the research results, it can be concluded that (1) the use of Moringa leaf extract can improve the growth of patchouli explants, and (2) the use of 40 grams/L of Moringa leaf extract can be an alternative to substitute the use of 1.5 ppm zeatin hormone.

Neem Oil to Reduce Zeatin Use and Optimize the Rooting Phase in Olea europaea L. Micropropagation

Abstract: Micropropagation is an in vitro propagation technique, established in the nursery field sector for numerous species, which offers several advantages compared to traditional agamic propagation techniques. In the case of the olive tree, however, despite the advances made through research, it is still little used, due to the recalcitrance to in vitro proliferation and/or rooting of many olive cultivars and the high cost of zeatin, the only cytokinin that makes it possible to achieve a satisfactory proliferation rate in this species. In this context, numerous attempts have been made to identify alternative cytokinin compounds able to improve the proliferation rate of olive tree explants and thus reduce the unitary production cost. In particular, there is a growing interest in the use of natural substances (called in some cases “complex mixtures”), which, when added to the in vitro cultivation substrates, seem to be able to improve proliferation rates. In the present study, neem oil was added to the propagation substrates (partially/totally replacing zeatin) and in the rooting phase for the olive cultivar Moraiolo. In particular, in the proliferation phase, the effect of neem oil (0.1 mL L−1) in substrates containing different zeatin concentrations (0, 1, 2, and 4 mg L−1) was evaluated. For the rooting phase, agarized substrate and soil were used with shoots derived from a standard proliferation substrate (4 mg L−1 zeatin) and from the substrate that gave the best results in the proliferation phase (2 mg L−1 zeatin and 0.1 mL L−1 neem oil). In the proliferation phase, the addition of neem oil in the substrates with low zeatin concentration (1 and 2 mg L−1) induced an increase in the number of adventitious shoots and shoots length. On the contrary, the addition of neem oil in the rooting substrates did not positively influence the rooting phase, but positive results especially in terms of root number and length were observed in explants derived from a neem oil-enriched proliferation substrate compared to the control substrate. Therefore, the present study demonstrated for the first time the positive role of neem oil in the proliferation of olive in vitro with low zeatin concentrations.

Transformed red pepper with agrobacterium and preparation method thereof

Abstract: PURPOSE: Provided is a method for preparing transformed red pepper using Agrobacterium tumefaciens LBA4404, which enables to introduce useful genes into crops. CONSTITUTION: A transformed red pepper is prepared by the following steps of: transforming red pepper cotyledon by using of Agrobacterium tumefaciens LBA4404; inducing shoot only from the transformed cotyledon tissue by cultivation in shoot-induction medium, which comprises MS(Murashige & Skoog) base medium including 3% of sucrose, 0.4% of gelrite, zeatin and indole acetic acid (IAA) as hormone, kanamycin and carbenicillin as antibiotics; cutting and serial passage culturing the shoot in shoot-elongation medium, which comprises MS(Murashige & Skoog) base medium including 3% of sucrose, 0.4% of gelrite, zeatin and NAA as hormone, kanamycin and carbenicillin as antibiotics to differentiate young plant; developing root by cultivation of the differentiated young transformant in root-induction medium, which comprises MS(Murashige & Skoog) base medium including 3% of sucrose, 0.4% of gelrite, IBA as hormone, kanamycin and carbenicillin as antibiotics.

Influence of Medium and Plant Growth Regulator on Micropropagation Efficiency in Blueberry

Abstract: The aim of this study was to develop an effective production system of blueberry plants by using tissue culture technique. Murashige and skoog medium (MS) and woody plant medium (WPM) were compared for shoot formation of highbush blueberries. Also medium supplemented with zeatin/2-isopentenyl adenine (2iP)/benzyl aminopurine (BA) (1, 2/10, 15/4, 6mg·L)and zeatin/2iP/BA (0.5/10, 15/0.05mg·L) as plant growth regulators to determine the effect of shoot formation and shoot proliferation, respectively. The shoot explants cultured on WPM showed higher shoot formation rates, more number of nodes, and longer root length than those on MS medium during the primary culture. Shoots were not formed when the explants were cultured on the medium without plant growth regulators or on only BA. The shoot explants cultured on the medium supplemented with 2iP showed low rates of shoot formation. On the other hand, zeatin was the most effective for shoot formation and growth of the explants. Also influence of different cytokinins (zeatin, 2iP) on the shoot proliferation of subcultured shoot explants was studied. There was no significant difference among the different concentrations of zeatin in the rate of shoot formation and number of shoots. However at higher concentration of zeatin, number of nodes was increased, and shoot length was shorted. The proper concentrations of zeatin for shoot propagation in subculture were found to be 0.5mg·L and 1mg·L. Additional key words : 2iP, BA, MS medium, WPM, zeatin