Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Production of progenitor cereal cells

Abstract: A process for the production and maintenance of pluripotent and/or totipotent progenitor cereal cells from undifferentiated callus cells is described. Production of the progenitor cells takes place via direct organogenesis on a medium containing at least one auxin and at least one cytokinin. For example, the auxin may be 2,4- dichlorophenoxyacetic acid, indole-3-acetic acid, picloran, naphthelenacetic acid, indole-3-propionic acid, indole-3-butyric acid, phenyl acetic acid, benzofuran-3-acetic acid or phenyl butyric acid, and the cytokinin may be benzyl amino purine, benzyladenine, thidiazuron, zeatin, isopentyladenine, trans-zeatin or dimethylallyladenine. Processes for transformation of the undifferentiated callus cells and/or the progenitor cereal cells are also described. Typical cereal cells are sorghum, maize, wheat, barley, millet, rye, canola, alfalfa, triticale and rice.

Tissue culture and rapid propagation method of blueberry

Abstract: The invention provides a tissue culture and rapid propagation method of blueberry. The method is characterized in that semi-lignified stems of the blueberry are used as explants to be inoculated to a culture medium containing improved READ culture medium, zeatin, naphthylacetic acid, saccharose and agar to perform connection culture; new tips are transplanted into a culture medium containing the improved READ culture medium, zeatin, saccharose and agar to perform continuous propagation culture; the regeneration seedlings are cut into small sections and then transplanted to a composite substrate, so as to achieve ex-vitro rooting. According to the method, zeatin is used as a plant growth modifier at the stages in a tissue culture vessel, including primary generation induction and subculture multiplication; zeatin has the effects of promoting cell division and cell growth, and is small in dosage and high in multiplication coefficients; the plants are strong and can be directly applied to the ex-vitro rooting process without strong sprout cultivation; meanwhile, the ex-vitro rooting technology is performed, which is high in rooting speed, high in efficiency, low in cost, and high in propagation speed compared with the test-tube rooting technology.

Transformation of potato plants with the isopentenyltransferase gene leads to enhanced stolon development and precocious tuber sprouting

Abstract: This study was conducted to show the effect of transformation of potato (Solanum tuberosum) plants with the isopentenyltransferase (ipt) gene on plant morphology and cytokinin levels in leaves and tubers. The ipt gene, which catalyzes the rate-limiting step of the cytokinin synthesis pathway, was placed under the control of a tuber-specific patatin promoter and introduced into potato plants (cv. Norchip) via Agrobacterium-mediated transformation. The presence of the ipt gene in transgenic potato plants was confirmed by Southern hybridization. Shoot morphology was normal in nearly all transgenic plants prior to and after tuberization. Transgenic plants had extensive stolon growth and an increased number of tubers. Premature sprouting of tubers and an increase in the number of sprouts were also observed in transgenic plants. Total fresh and dry weight of tubers was similar in control and transgenic plants. Expression of the ipt gene was tuber specific, as determined by northern hybridization and cytokinin analysis. The concentration of zeatin and zeatin riboside was increased in tubers of transformed plants, but the level in source leaves was similar in transformed and control plants. This study supports a role for cytokinin in tuber initiation and release of dormancy.

Effect of Rootstock, Buds Soaking in Benzyl Adenine and Foliar Spray of Brassinolide on The Chemical and Hormonal Content of Produced Orange Transplants

Abstract: This experiment was carried out in the lath house of the department of Horticulture , landscaping / college of Agriculture / University of Baghdad during 10/04/2012 to 01/11/2013. Designed in accordance with R.C.B.D used with three replicate to study the effect of the impact local oranges soaking regulator BA concentrations (0,100,300) Mg.l -1 on three rootstocks of citrus (sour orange, volkamer lemon, Cleopatra mandarin), and after the success of the grafts is sprayed seedlings plant growth regulator concentrations Brassinolide (0.005,0.003,0) Mg.l -1 in the chemical and hormonal content of seedlings Done data analysis according to the statistical program sas averages were compared using the test Duncan’s multiple range at the possibility of 5%. The results can be summarized as follows: The study showed that it was not out of the impact on securities content of nitrogen and phosphorus, it has had a significant effect in the papers content of potassium content hormonal as continued volkamer lemon excellence in securities content of potassium and Zeatin as it gave content was 1.34%, 42.95, while superiority continued tangerine Cleopatra in Securities and carbohydrate content of Brassinolide and auxin and ghebrelin, amounting to 3.17% (28.04, 25.32, 34.5) -1 Maekerowerem.gram sequentially, Either on the impact of soaking Pal BA is observed superiority focus 300 Mg.l -1 giving him a higher content of carbohydrates, nitrogen, phosphorus, potassium and Brassinolide and Auxin and Alghebrelin as it stood at 3.29%, 1.64%, 0.40%, 1.40%, (32.83, 24.67, 34.76) Maekerowerem.gram -1 sequentially, while the comparison given treatment less content of the qualities mentioned above. The effect of spraying Brassinolide Fimaa_khas was found superiority focus 0.005 Mg.l -1 morally and gave the highest content of carbohydrates and Zeatin and Brassinolide and Auxin and Alghebrelin, amounting to 3.21% (41.58, 33.41, 24.40, 41.72) -1 Maekerowerem.gram sequentially, while Attt comparison treatment less content of the recipes mentioned Anfa.ama on bilateral interactions and triple overlap there were significant differences as a result of these interactions.

An Efficient Method for Adventitious Shoot Regeneration from Leaf and Stem-segment Explants of Russian Olive Elaeagnus angustifolia L.

Abstract: The establishment of an efficient adventitious shoot regeneration system from leaf discs and stem segments of one-year-old Russian olive(Elaeagnus angustifolia L.) was established.Shoot regeneration using leaf explants and stem segments was obtained on MS medium supplemented with different cytokinins viz.,6-benzyladenine(BA) and Zeatin(Zt),with combination of auxins viz.,naphthaleneacetic acid(NAA) andindole-3-butyric acid(IBA).MS + 0.5mg/L 6-BA + 0.2mg/L NAA was found to be a better combination for leaf regeneration,producing a maximum of 4.3 shoots per explant,whereas MS + 1.0mg/L Zt + 0.5mg/L NAAfor stem-segment explants produced the highest number of multiple shoots(3.6) per explant.The regenerated shoots produced 100% rooting on 1 /2 strength MS medium supplied with 0.5mg/L NAA.In vitro raised plantletswere successfully transferred to pots containing sterile soil∶ peat moss∶ sand(1∶ 1∶ 1) for acclimatization,and 77% of rooted plantlets survived under ex situ conditions.The currently employed regeneration protocol through leaf explants and stem segments facilitates germplasm conservation of this medicinally and ecologically importantspecies besides providing a standardized regeneration protocol for genetic transformation studies in the future.