Topic
Zeatin
About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.
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TL;DR: Of the shoot primordia obtained, those cultured on WP medium supplemented with 10μM IAA and 30μM 2iP showed the most active proliferation, being 2-4 times in size, after one month of subculture.
Abstract: A mass of shoot primordia were induced effectively from the shoot apex of Rhododendron pentaphyllum Maxim. var. Komatsu on WP media containing 30μM 2iP+IAA (10, 30, 100μM). Of the shoot primordia obtained, those cultured on WP medium supplemented with 10μM IAA and 30μM 2iP showed the most active proliferation, being 2-4 times in size, after one month of subculture. When shoot primordia obtained were transferred onto the differentiation media, multiple shoots were formed on WP media containing zeatin (1, 10μM) or 0.1μM CPPU after one month of culture, irrespective of the presence of IAA. The multiple shoots induced on the medium containing 10μM IAA and 10μM zeatin showed active elongation growth. Rooting from the multiple shoots subcultured on the same fresh medium occurred two months after the shoot differentiation.
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TL;DR: Cytokinin-autotrophic strains of carrot callus contained active substances with Chromatographic mobilities on Sephadex LH-20 corresponding to 6-(4-hydroxy-3-methylbut-trans-2-enylamino)purine (Zeatin, Z), 6-(3- methylbut- 2-eny Lamino) purine (iP) and the ribosides (9R)Z and (9P)iP, indicating
Abstract: Cytokinin-autotrophic strains of carrot callus contained active substances with Chromatographic mobilities on Sephadex LH-20 corresponding to 6-(4-hydroxy-3-methylbut-trans-2-enylamino)purine (Zeatin, Z), 6-(3-methylbut-2-enylamino)purine (iP) and the ribosides (9R)Z and (9R)iP. The apparent major activity was found in a fraction, with an elution volume of 242–291 ml. Hydrolysis of this fraction with HCl and Β-Glucosidase gave rise to Z, indicating that the major active compound is a polar conjugate of Zeatin.
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