Topic
Zeatin
About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.
Papers published on a yearly basis
Papers
More filters
TL;DR: It is concluded that the suspensor plays an essential role in embryogenesis by acting as a hormone source to the early embryo.
Abstract: Data are presented on the cytokinin status of seeds and seed components, at different stages of development in Phaseolus coccineus L., as determined with the soybean callus growth bioassay: A change in cytokinin types according to developmental stage occurred: from biologically very active less polar types (zeatin=Z) at early stages to more polar types (zeatin glucoside=Z9G and zeatin riboside=Zr), with relatively low biological activity, at intermediate and late stages of seed development: When cytokinins were analyzed separately in embryos (embryo proper) and suspensors at two embryonic stages: heart-shaped (A) and middle cotyledonary embryos (stage B) respectively, it was found that: i) at stage A, the suspensor showed cytokinin activity at the level of Z, 2iPA (2-isopentenyladenosine) and Zr, whereas more polar cytokinins (Z9G, Zr) were present in the embryo; ii) at stage B, when the embryo seems to become autonomous for cytokinin supply, there was a relative abundance of active cytokinins (Z, 2iPA) in the embryo to which Z9G activity in the suspensor corresponded. It is concluded that the suspensor plays an essential role in embryogenesis by acting as a hormone source to the early embryo.
56 citations
TL;DR: Five cytokinin activities which induced soybean callus proliferation were detected in ethanol extracts of root nodules of the garden pea and the most active factors were identified as zeatin and its riboside on the basis of their mobility on thin layer chromatography in three solvent systems.
Abstract: Five cytokinin activities which induced soybean callus proliferation were detected in ethanol extracts of root nodules of the garden pea (Pisum sativum L., cv. Little Marvel). The most active factors among them were identified as zeatin and its riboside on the basis of their mobility on thin layer chromatography in three solvent systems. Smaller activities of zeatin ribotide, isopentenyladenine and its riboside were also detected. Cytokinin activity gradually decreased with the cultivation period, but no qualitative change in the active compounds was found.
56 citations
TL;DR: Plant regeneration has been achieved routinely from established cell suspension culture lines of Vigna aconitifolia (moth bean), a highly drought tolerant grain legume, from three-week-old leaf callus.
Abstract: Plant regeneration has been achieved routinely from established cell suspension culture lines of Vigna aconitifolia (moth bean), a highly drought tolerant grain legume. The cultures originated from three-week-old leaf callus. Several media including MS, B5, AA, SL, PCM, SH and L-6 were tested for their effects on cell growth. Maximum growth was observed in L-6 medium containing 44.5 μM 2,4-D. After 6 to 8 weeks the suspensions were filtered through 500, 250, 125 and 60 μm sieves, respectively, for four to five subcultures. An embryogenic cell line (VA-686) was obtained from the cell fraction collected below 250 μm. The VA-686 cell line is being maintained on L-6 medium with 4.5 μM 2,4-D and 2.3 μM Zeatin. Somatic embryogenesis was induced by transferring the cells to L-6 medium with 4.6 μM zeatin in which green cell clusters were produced. The somatic embryos developed from most of the cell clusters when plated on L-6 agar medium with 2.3 μM BA. Plantlets were obtained from the embryos on L-6 medium with 10.0 μM IBA. The regenerated plants were grown to maturity in the greenhouse.
56 citations
TL;DR: Ammonium nitrate induced retardation of leaf senescence may be mediated at least partly by its effect on foliar cytokinin content, particularly in growing tobacco leaves excised from near the apex of the plant.
Abstract: The effect of nitrogenous nutrients on endogeneous cytokinins and senescence of tobacco leaves was investigated. Ammonium nitrate was the most effective in retarding senescence and its activity was attributed principally to NH4+ ions. Repeated applications or a continuous supply of ammonium nitrate was required for maximal retardation of tobacco leaf senescence. Ammonium nitrate solution supplied via the petioles reduced the senescence retarding effect of dihydrozeatin applied directly to the laminae of detached tobacco leaves. Ammonium nitrate also elevated the endogenous levels of cytokinins (especially zeatin and dihydrozeatin) particularly in growing tobacco leaves excised from near the apex of the plant. Ammonium nitrate induced retardation of leaf senescence may be mediated at least partly by its effect on foliar cytokinin content.
56 citations
TL;DR: Interestingly, as identified by MS/MS, very sharp and transient premitotic peak of cytokinins contained, in addition to trans -zeatin, also considerable amounts of cis-zeatin and zeatin- O -glucoside.
56 citations