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Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.


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Journal ArticleDOI
TL;DR: In vitro protocols for plantlet regeneration and medium-term genotype conservation of eight wild species of Curcuma have been optimized and benzyladenine was found superior to other cytokinins tested for plant let regeneration and γ,γ-dimethylallylaminopurine for conservation.
Abstract: In vitro protocols for plantlet regeneration and medium-term genotype conservation of eight wild species of Curcuma have been optimized. Both the phenomena were genotype-dependent and influenced significantly by type and concentration of cytokinins used. In general, benzyladenine (BA) was found superior to other cytokinins tested for plantlet regeneration and γ,γ-dimethylallylaminopurine (2iP) for conservation. Number of shoots per culture ranged from 1.3 to 7.2 and conservation period from 264 to 379 d. In 30-d-old cultures, highest frequency of shoot regeneration could be obtained in C. malabarica (7.2 shoots per culture) on MS + 11.4 μM zeatin. Curcuma sp. (unidentified wild species) could be conserved for maximum period (379 d) on MS + 24.6 μM 2iP followed by C. aromatica (363 d) on MS + 22.8 μM zeatin. The tissue culture-raised plantlets were morphologically similar to their parents. The in vitro-conserved plants multiplied rapidly in tissue cultures and produced normal rhizomes upon transfer to soil in net house.

53 citations

Journal ArticleDOI
TL;DR: The relationship of assimilate supply to grape (Vitis vinifera L.) berry growth and development was studied with a seeded ('Kyoho') and a seedless ('Seedless Wuhehongʼ) cultivar as discussed by the authors.
Abstract: The relationship of assimilate supply to grape (Vitis vinifera L.) berry growth and development was studied with a seeded ('Kyohoʼ) and a seedless ('Seedless Wuhehongʼ) cultivar. A single shoot girdling between the second and third nodes below the basal cluster at the end of Stage I of berry growth shortened Stage II (the lag phase) of 'Kyohoʼ grape berries by 10 days, and eliminated Stage II in 'Seedless Wuhehongʼ grape berries. Double shoot girdling between the second and third nodes below the basal cluster and above the upper cluster, respectively, at the same time at the end of Stage I, advanced Stage II by 3 days in both cultivars. Normal accumulation of dry weight in the 'Kyohoʼ grape berry is in a double sigmoidal pattern, but it became a single sigmoidal pattern in response to a single basal girdling. The highest percent moisture in berries was at 20 days after full bloom. Rapid changes in berry pectin substances lagged behind those of soluble solids and titratable acidity, and behind the onset of berry softening at veraison in 'Kyohoʼ, but not in 'Seedless Wuhehongʼ, for which the three processes were con cur rent. It is suggested that the slow growth of the berries during Stage II is a result of a decrease in the rate of water accumulation on a whole berry basis and a decrease in accumulation of dry matter in the skin and fl esh (pericarp) due to assimilate com pe ti tion within grapevines and within berries. The relationships between levels of endogenous hormones (IAA, GA3, zeatin, zeatin riboside, and ABA) and berry growth were also studied with 'Kyohoʼ grapes. The results showed that the slow growth of grape berries during Stage II was associated with assimilate com pe ti tion between the skin-fl esh (pericarp) and seeds, and with peak shifts of concentrations of IAA, GA3, zeatin and zeatin riboside. Changes in ABA levels were closely associated with ripening and senescence during late Stage III. has been paid to the ripening process with the suggestion that the auxin-ABA relationship might be involved in the hormonal regulation of veraison and ripening of grape berries.

53 citations

Journal ArticleDOI
TL;DR: Results from substrate competition experiments and enzyme separation by anion exchange HPLC indicate a single, distinct, zeatin O-glycosylation enzyme occurs in embryos of each of these Phaseolus species.
Abstract: An enzyme catalyzing the formation of O-glucosylzeatin in immature embryos of Phaseolus lunatus was purified 2500-fold using ammonium sulfate precipitation followed by affinity and anion exchange chromatography. The enzyme uses trans-zeatin as substrate (K(m) 28 micromolar) but not cis-zeatin, ribosylzeatin, or dihydrozeatin. Both UDP-glucose and UDP-xylose can serve as glycosyl donors, with K(m)s of 0.2 and 2.7 millimolar, respectively, for the formation of O-glucosylzeatin and O-xylosylzeatin. In comparison, the UDPxylose-zeatin:O-xylosyltransferase (JE Turner, DWS Mok, MC Mok, G Shaw [1987] Proc Natl Acad Sci USA 84: 3714-3717) isolated by the same procedures from P. vulgaris embryos uses only UDP-xylose as donor substrate and the K(m)s for both zeatin and UDP-xylose are much lower (2 and 3 micromolar, respectively). The chromatographic behavior on affinity columns and molecular weights (approximate M(r) 44,000 daltons) of the two enzymes are similar. Results from substrate competition experiments and enzyme separation by anion exchange HPLC indicate a single, distinct, zeatin O-glycosylation enzyme occurs in embryos of each of these Phaseolus species.

53 citations

Journal ArticleDOI
TL;DR: Honeydew and leaf extracts from Salix babylonica indicate that large quantities of cytokinin are present in the leaves and are transported through the phloem of this plant during late autumn.
Abstract: Honeydew and leaf extracts from Salix babylonica indicate that large quantities of cytokinin are present in the leaves and are transported through the phloem of this plant during late autumn The active compound in the extracts could be hydrolysed with β-glucosidase, whereafter it showed the same chromatographic behaviour as zeatin It is proposed that cytokinins in the leaves are converted to the glucoside and then redistributed to the rest of the plant organs where it is stored

53 citations

Journal ArticleDOI
TL;DR: Adventitious shoots were regenerated from axillary bud explants of 15 carnation cultivars but the use of leaf and stem explants was not succesful, largely due to explant senescence in the presence of benzyladenine, kinetin and, to a lesser extent zeatin.

53 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202333
2022103
202135
202034
201932
201848