Phosphorus Assay in Column Chromatography

Microdetermination of Phosphorus

https://helvia.uco.es/xmlui/bitstream/handle/10396/10963/Spectrophotometric%20Quantitation%20%281%29.pdf?sequence=1

Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E.

The Regulation of Rabbit Skeletal Muscle Contraction I. BIOCHEMICAL STUDIES OF THE INTERACTION OF THE TROPOMYOSIN-TROPONIN COMPLEX WITH ACTIN AND THE PROTEOLYTIC FRAGMENTS OF MYOSIN

Naphthalenesulfonamides such as N-(6-amino-hexyl)-5-chloro-1-naphthalenesulfonamide (W-7) are potent calmodulin (CaM) antagonists and act upon several protein kinases at higher concentration. When the naphthalene ring was replaced by isoquinoline, the derivatives were no longer CaM antagonists but retained the ability to inhibit protein kinases, and some of the derivatives exhibited selective inhibition toward a certain protein kinase. cAMP-dependent, cGMP-dependent, and Ca2+-phospholipid-dependent (protein kinase C) protein kinases were inhibited significantly by addition of 10(-6) M N-[2-(methylamino)ethyl]-5-isoquinoline-sulfonamide (H-8) and 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7). H-8 was the most active of the inhibitors in this series and inhibited more markedly cyclic nucleotide dependent protein kinases, than other kinases, while the derivative with the sulfonylpiperazine residue (H-7) was the most potent in inhibiting protein kinase C. Apparent Ki values of H-8 were 0.48 and 1.2 microM for cGMP-dependent and cAMP-dependent protein kinases, respectively, and the Ki value of H-7 for protein kinase C was 6 microM. Both the holoenzyme and the catalytic subunit (or fragment), which is active without an enzyme activator, are susceptible to these compounds with a similar concentration dependency, thereby indicating that the inhibitory effect is attributed to the direct interaction of the compound with the active center of the enzyme but not with the enzyme activator. The inhibitions were freely reversible and of the competitive type with respect to ATP and of the noncompetitive type with respect to the phosphate acceptor.(ABSTRACT TRUNCATED AT 250 WORDS)

Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C.

The colorimetric determination of phosphorus

http://science.sciencemag.org/content/sci/65/1686/401.full.pdf

The nature of the "inorganic phosphate" in voluntary muscle.

The first very important work of SubbaRow with Fiske was to develop a rapid but accurate method for the determination of phosphorus as phosphate in biological samples. It paved the way for establishing the presence of several other biological phosphorus compounds. They discovered phosphocreatine followed quickly by ATP, glycerophosphate and others. Here we have reproduced the paper wherein they have described their work on muscle ATP, a significant contribution to DNA structure, and liver glycerophosphate.

https://science.sciencemag.org/content/sci/70/1816/381.2.full.pdf

Phosphorus compounds of muscle and liver

A study has been made of the nutritional requirements of the pneumococcus. The relatively simple, chemically-defined medium composed of glutathione, thiochrome, nicotinamide, betaine, flavin, glucosamine, uracil, guanylic acid, xanthine, hypoxanthine, pantothenic acid, gelatin hydrolysate, amino acids, inorganic salts, and glucose which can support the growth of the Dochez NY 5 strain of hemolytic streptococcus (Rane and Subbarow, 1938; Subbarow and Rane, 1939) was found to be deficient for pneumococci. The addition of a highly-active, purified extract of liver provided conditions suitable for good growth of certain types of Pneumococcus. One fraction isolated from this liver extract demonstrated the growth value of a compound similar to, and replaceable by choline. Satisfactory growth was subsequently obtained in a medium consisting of gelatin hydrolysate, certain additional amino acids, inorganic salts, glucose, choline, nicotinic acid, pantothenic acid, flavin, and thioglycollic acid. In some cases a mixture of known amino acids could be substituted for the gelatin hydrolysate.

Nutritional Requirements of the Pneumococcus: I. Growth Factors for Types I, II, V, VII, VIII

http://science.sciencemag.org/content/sci/67/1728/169.full.pdf

Y. Subbarow

Papers

The colorimetric determination of phosphorus

The nature of the "inorganic phosphate" in voluntary muscle.

Phosphorus compounds of muscle and liver

Nutritional Requirements of the Pneumococcus: I. Growth Factors for Types I, II, V, VII, VIII

The isolation and function of phosphocreatine