Determination of serotonin and dopamine in mouse brain tissue by high performance liquid chromatography with electrochemical detection

https://mmbr.asm.org/content/mmbr/33/2/264.full.pdf

Mechanism of protein biosynthesis.

Homogeneous preparations of initiation factor F3 have both RNA binding and ribosome dissociation activities. But although DF activity could entirely account for the role of F3 in the ribosome cycle this factor has an additional function in formation of the initiation complex that may be related to its RNA binding properties.

Purification and Properties of Initiation Factor F3

In this article Dr Davis reviews recent developments in the search for the precise nature of the cycle of ribosomes in protein synthesis.

Role of Subunits in the Ribosome Cycle

The following two articles clarify the involvement of initiation factor F3 in the translation of messenger RNA. First, Sabol and Ochoa tell how they used 35S-labelled F3 to prove that 70S ribosomes, released at polypeptide chain termination, are dissociated when F3 binds to the 30S ribosomal subunit.

Ribosomal binding of labelled initiation factor F3.

PREVIOUS work has demonstrated that the initiation of protein synthesis requires messenger RNA (mRNA)1–3, formyl-methionyl-tRNA (F-met-tRNA)4–6, ribosomes, GPT7–9, and at least three protein initiation factors10–12. Native ribosomal subunits have been shown to contain the initiation factors, and such subunits are extremely active in the initiation of protein synthesis13.

A. Eisenstadt

Papers

Isolation of protein initiation factors from 30S ribosomal subunits.