Background: Urinary Schistosomiasis (US) caused by Schistosoma haematobium constitutes a major public health problem in Nigeria. Efforts in this study were devoted to evaluate the current prevalence and associated risk factors on the intensity of disease burden. Methods: A survey was conducted with the use of semi-structured questionnaires to collect sociodemographic and anthropometric data of the pupils. Urine samples were collected from One hundred and two (102) pupils for microscopic analysis for the detection of S.haematobium eggs. Results: Of the 102 children (age ranges between 5-15 years) examined, prevalence of infection was (80/102) 78 % of which (44/102) 43 % were heavy and (36/102) 35 % mild while (22/102) 22 % were negative (p washing of clothes in streams > playing / bathing in rivers> unknown > mosquito bites > fresh water snail. No significant difference exists between the risk factors (p>0.05). The mean age, weight, height, waist circumference and hip circumference of the negative individuals (both males and females) were significantly higher than that of their positive counterpart (p 0.05). Similarly, there was no significant difference in the mean hip circumference of the heavy and mild infected male and female (p>0.05). Correlation analysis showed that there is significant correlation between infection status and age (r= 0.385, p>0.01), weight (r = 0.404, p>0.01), height (r=0.401, p>0.01), waist (r=0.396, p>0.01), hip circumference (r=0.415, p>0.01) and risk factor (r=-0.231, p>0.05). Risk factor is not significant to age, weight, height, waist, hip circumference and infection status (p>0.05). Conclusion: Prevalence rate obtained in the study was high, an indication that the region is endemic by WHO classification. This calls for an urgent effective urinary schistosomiasis control programme in the zone.

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Prevalence and risk factors of Schistosoma haematobium infections among primary school children in Igbokuta Village, Ikorodu North Local Government, Lagos State.

https://www.ajol.info/index.php/aju/article/download/163833/153305

Prevalence of urinary schistosomiasis in Nigeria, 1994–2015: Systematic review and meta-analysis

Schistosoma haematobium, a major pathogen of urogenital schistosomiasis, has been reported to be affecting an estimated 30 million people in Nigeria. Current national estimates of S. haematobium an...

Epidemiological dynamics and associated risk factors of S. haematobium in humans and its snail vectors in Nigeria: a meta-analysis (1983-2018).

Introduction: Schistosoma haematobium infection afflicts about 150 million people in 53 countries in Africa and the Middle East. In many endemic areas, S. haematobium is sympatric with Schistosoma bovis, Schistosoma mattheei, Schistosoma curassoni, Schistosoma intercalatum and Schistosoma magrebowiei, its closely related species. In addition, they also develop in the same intermediate snail hosts. Since these schistosome species often infect snails inhabiting the same bodies of water, examining cercariae or infected snails for estimating transmission of S. haematobium is always confounded by the need to differentially identify S. haematobium from these other species. Recently, differentiating S. haematobium by polymerase chain reaction (PCR) from S. bovis, S. mattheei, S. curassoni and S. intercalatum, but not from S. magrebowiei was reported. However, to be able to evaluate residual S. haematobium transmission after control interventions in areas where S. haematobium may be sympatric with S. magrebowiei, a differential tool for accurate monitoring of infected snails is needed. Materials and Methods : Thus in this study, we developed a new PCR assay using a pair of primers, ShND-1/ShND-2, to amplify a target sequence of 1117 bp (GenBank accession number KF834975) from S. haematobium mitochondrion complete genome (GenBank accession number DQ157222). Sensitivity of the assay was determined by PCR amplification of different concentrations of S. haematobium gDNA serially diluted from 10ng to 0.1pg. For assay specificity, different concentrations of gDNA from S. haematobium and the other schistosome species, 20 positive urine samples and five controls as well as 20 infected snails were subjected to PCR amplification, while some of the PCR products were sequenced. Results : The assay detected up to 1pg of S. haematobium gDNA, while a differential identification of S. haematobium DNA content from other closely related species was achieved when applied to urine and naturally infected snails. When a protein-protein blast search was carried out using Blastp, the amplified sequence was found to encode a protein that shows a 100% similarity with S. haematobium nicotinamide adenine dinucleotide dehydrogenase subunit 3 (GenBank accession number YP_626524.1). Conclusion : The PCR assay was sensitive, specific and was able to successfully differentiate S. haematobium from S. magrebowiei, in addition to its other closely related animal infective schistosome species.

Differentiating Schistosoma haematobium from Schistosoma magrebowiei and other closely related schistosomes by polymerase chain reaction amplification of a species specific mitochondrial gene.

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The Sub-Saharan Water Crisis: An Analysis of its Impact on Public Health in Urban and Rural Nigeria

Background: A survey of Schistosoma haematobium infection in Epe, an urban community in Lagos State, Southwest Nigeria, was carried out to ascertain the possibility that schistosomiasis, otherwise considered a rural disease, could reach urban populations. Materials and Methods: About 100 ml of voided urine samples from 200 pupils aged 6-13 years [109 (54.5%) males and 91 (45.5%) females], attending an Anglican primary school, Ebute Afuye, and a community primary school, Erepoto, were examined parasitologically for hematuria and S. haematobium ova following informed consent obtained from their parents/guardians. All samples were screened using polymerase chain reaction (PCR) amplification of the schistosome Dra1 gene. Fourteen Bulinus snails collected from the two sites, Ebute Afuye (6) and Erepoto (8), were screened for schistosome infection by the PCR amplification of the schistosome Dra1 gene. PCR-RFLP of the snails' its region was analyzed for species identification and a subregion of the cox1 gene from four infected snails (two from each site) was amplified and sequenced. Results: In the Anglican primary school, Ebute Afuye, and community primary school, Erepoto, 16% and 29% were positive for hematuria, and 16% and 17% had schistosome ova, respectively. PCR analysis showed that 57% and 40% were positive for the infection in Anglican primary school, Ebute Afuye, and community primary school, Erepoto, respectively. PCR screening of the snails confirmed that four from Ebute Afuye and three from Erepoto were infected with schistosomes. PCR-RFLP showed that all the 14 snails were Bulinus truncatus while phylogenetic analysis of the sequenced partial cox1 gene corroborated the PCR-RFLP results. Conclusions: There was a high prevalence of S. haematobium infection among the participants detected by PCR, which was able to detect infection in cases otherwise shown to be negative by hematuria. We also observed that B. truncatus is one of the snail species responsible for the transmission of urinary schistosomiasis in the Epe community. For national control programs, it is very important that trends in the prevalence and intensity of schistosomiasis in urban cities be monitored.

AA Dike

Papers

Urinary schistosomiasis transmission in Epe, an urban community of Southwest Nigeria