Recent developments in quantification methods for metallothionein.

Binding of copper and zinc to three cyanobacterial microcystins quantified by differential pulse polarography

Electrochemical study of a metallothionein modified gold disk electrode and its action on Hg2+ cations

The main aim of this work was to prepare and determine apoMT by
adsorptive transfer stripping technique (AdTS) in connection
with differential pulse voltammetry (DPV). Particularly, we
investigated the electrochemical behavior of MT measured on the
surface of hanging mercury drop electrode in the presence of
sodium chloride as supporting electrolyte using AdTS DPV with
respect to study the effects of MT signals by different
concentrations and pH's of the electrolyte. Then, we aimed at
utilizing this technique to observe changes in MTwhich are
dependent on using strong chelating compounds ethylendiamine-N,
N, N', N'-tetraacetic acid (EDTA). Thanks to ability of EDTA to
bind heavy metals from active center of enzymes and regulation
proteins, we were able to prepare apoMT; that means, MT without
any metal ion bound in its structure. Detection limit of apoMT
at very short time of accumulation (tA 120 s) was 3 nM (20
ng/mL and/or 15 fmol in 5 mL drop; RSD 2 -- 5%) estimated by
dilution of the analyzed solution until the signal disappeared.
In addition, it was possible to decrease detection limit by
extending of time of accumulation of apoMT on the surface of
HMDE. We were able to detect 30 pM of apoMT (200 pg/mL) at
tA500 s.

/pdf/determination-of-apo-metallothionein-using-adsorptive-4umkfkfu37.pdf

Adela Rosa Rodriguez

Papers

The electrochemical behaviour of Cd,Zn thioneins depending on the solution pH using differential pulse polarography