The influence of diet on the distribution of nitrogen isotopes in animals was investigated by analyzing animals grown in the laboratory on diets of constant nitrogen isotopic composition. 
The isotopic composition of the nitrogen in an animal reflects the nitrogen isotopic composition of its diet. The δ^(15)N values of the whole bodies of animals are usually more positive than those of their diets. Different individuals of a species raised on the same diet can have significantly different δ^(15)N values. The variability of the relationship between the δ^(15)N values of animals and their diets is greater for different species raised on the same diet than for the same species raised on different diets. Different tissues of mice are also enriched in ^(15)N relative to the diet, with the difference between the δ^(15)N values of a tissue and the diet depending on both the kind of tissue and the diet involved. The δ^(15)N values of collagen and chitin, biochemical components that are often preserved in fossil animal remains, are also related to the δ^(15)N value of the diet. 
The dependence of the δ^(15)N values of whole animals and their tissues and biochemical components on the δ^(15)N value of diet indicates that the isotopic composition of animal nitrogen can be used to obtain information about an animal's diet if its potential food sources had different δ^(15)N values. The nitrogen isotopic method of dietary analysis probably can be used to estimate the relative use of legumes vs non-legumes or of aquatic vs terrestrial organisms as food sources for extant and fossil animals. However, the method probably will not be applicable in those modern ecosystems in which the use of chemical fertilizers has influenced the distribution of nitrogen isotopes in food sources. 
The isotopic method of dietary analysis was used to reconstruct changes in the diet of the human population that occupied the Tehuacan Valley of Mexico over a 7000 yr span. Variations in the δ^(15)C and δ^(15)N values of bone collagen suggest that C_4 and/or CAM plants (presumably mostly corn) and legumes (presumably mostly beans) were introduced into the diet much earlier than suggested by conventional archaeological analysis.

Influence of Diet On the Distribtion of Nitrogen Isotopes in Animals

Nitrate (NO3 ) concentrations in public water supplies have risen above acceptable levels in many areas of the world, largely as a result of overuse of fertilizers and contamination by human and animal waste. The World Health Organization and the U.S. Environmental Protection Agency have set a limit of 10 mg L nitrate (as N) for drinking water because nitrate poses a health risk, especially for children, who can contract methemoglobinemia (blue-baby syndrome). Nitrate in lower concentrations is non-toxic, but the risks from long-term exposure are unknown, although nitrate is a suspected carcinogen. High concentrations of nitrate in rivers, lakes, and coastal areas can cause eutrophication, often followed by fi sh-kills, due to oxygen depletion. Increased atmospheric loads of anthropogenic nitric and sulfuric acids have caused many sensitive, low-alkalinity streams in North America and Europe to become acidifi ed. Still more streams that are not yet chronically acidic could undergo acidic episodes in response to large rain storms and/or spring snowmelt, seriously damaging sensitive local ecosystems. Future climate changes may exacerbate the situation by affecting biogeochemical controls on the transport of water, nutrients, and other materials from land to freshwater ecosystems. The development of effective management practices to preserve water quality, and remediation plans for sites that are already polluted, requires the identifi cation of actual N sources and an understanding of the processes affecting local nitrate concentrations. In particular, a better understanding of hydrologic fl owpaths and solute sources is required to determine the potential impact of contaminants on water supplies. Determination of the relation between nitrate concentrations in groundwater and surface water and the quantity of nitrate introduced from a particular source is complicated by:

Tracing Anthropogenic Inputs of Nitrogen to Ecosystems

▪ Abstract Aquatic insects and other benthic invertebrates are the most widely used organisms in freshwater biomonitoring of human impact. Because of the high monetary investment in freshwater management, decisions are often based on biomonitoring results, and a critical and comparative review of different approaches is required. We used 12 criteria that should be fulfilled by an “ideal” biomonitoring tool, addressing the rationale, implementation, and performance of a method. After illustrating how the century-old but still widely used Saprobian system does not meet these criteria, we apply them to nine recent approaches that range from the suborganismal to the ecosystem level. Although significant progress has been made in the field, no recent approach meets all 12 criteria. Given that the use of biomonitoring information has important financial consequences, we suggest that societies and governments prioritize how these criteria should be ranked.

DEVELOPMENTS IN AQUATIC INSECT BIOMONITORING: A Comparative Analysis of Recent Approaches

Many natural streams are net heterotrophic, so I propose that trophic state be divided into autotrophic and heterotrophic state. This division allows consideration of the influence of external carbon sources as well as nutrients such as nitrogen and phosphorus. Empirical results suggest that phosphorus and nitrogen are the most important nutrients regulating autotrophic state in flowing waters and that benthic algal biomass is positively correlated to gross primary production in streams. Reference (minimally influenced by human activities) nutrient concentrations and correlations of nutrients with algal biomass are used to characterize reference distributions of stream autotrophic state. Only when reference nutrient concentrations are in the upper one third of those expected in the United States, is maximum benthic chlorophyll projected to exceed 100 mg m 22 (a concentration commonly used to indicate nuisance levels) .30% of the time. Average reference nutrient concentrations lead to sestonic chlorophyll concentrations above those considered typical of eutrophic lakes (. 8m g m 23 ) less than half the time. Preliminary analysis suggests that autotrophic state is variable in small pristine streams because it is influenced by canopy cover (light), but heterotrophic state is less variable because it can be based on allochthonous or autochthonous production. Nitrogen and phosphorus enrichment can influence both heterotrophic and autotrophic state, and these effects could cascade to animal communities. Stoichiometry should be considered because carbon, nitrogen, and phosphorus are all involved in trophic state. The proposed definition of trophic state offers a starting conceptual framework for such considerations.

/pdf/eutrophication-and-trophic-state-in-rivers-and-streams-52qihaqtzf.pdf

Eutrophication and trophic state in rivers and streams

Stable Isotope Tracing of Temporal and Spatial Variability in Organic Matter Sources to Freshwater Ecosystems

Fresh waters worldwide receive substantial loadings of particulate organic waste from municipal sewage collection and treatment works. This material may transport a wide variety of particle-associated contaminants. Using stable isotopes of carbon and nitrogen, we detected the influence of a nearby primary sewage treatment plant on the benthic food web of a large temperate river. Despite the low concentration of wastewater and the absence of accumulated sediment, >60% of carbon and nitrogen in benthic organisms near the outfall was of sewage origin. This fraction is disproportionately high relative to the fraction of sewage-derived suspended particulate matter in the seston, suggesting preferential assimilation. Assimilation of sewage-derived particulate organic matter exposes benthos to high levels of contamination and may represent an important source of contaminants to higher trophic levels.

Quantifying assimilation of sewage-derived organic matter by riverine benthos

We evaluated the role of sewage as a resource for the littoral food web of the fluvial St. Lawrence River near Montreal, Quebec. Stable isotope analysis indicated that macroinvertebrate primary con...

http://people.uleth.ca/~joseph.rasmussen/pdfs/deBruyn-etal2003.pdf

The role of sewage in a large river food web

We used two approaches (interspecific upper bound and normalized biomass spectrum, NBS) to study size distributions in a littoral fish community across a natural gradient of epiphytic primary production and at sites enriched by primary-treated sewage. The upper bound of the interspecific density  body size relationship supported universal energetic constraints on the regional assemblage but revealed substantial variation in size distributions at a local scale. Multivariate analysis of the modes composing the NBS suggested possible mechanisms for this local variation in size distributions. Only the largest organisms had higher densities at higher levels of productivity. Densities of smaller organisms could be explained by a combination of habitat characteristics and feeding interactions.

http://people.uleth.ca/~joseph.rasmussen/pdfs/deBruyn-etal2002.pdf

Altered body size distributions in a large river fish community enriched by sewage

Summary 1. We studied the response of the St Lawrence River food web to enrichment by primarytreated sewage from the city of Montreal, Canada. 2. Despite the richness and complexity of this food web, the openness of the system and the limited production possible in this temperate river’s short growing season, we found that biomass densities of consumers responded to enrichment according to the qualitative predictions of a simple bioenergetic model. 3. All consumer trophic levels responded to sewage enrichment, but not all members of each trophic level: top piscivores seemed to control small, benthivorous fish but were unable to control larger fish, so the intermediate consumer level became dominated by Catastomidae (suckers). Consequently, the epiphytic prey of small fish were free to respond to sewage enrichment, but the benthic prey of suckers were not. 4 . The important elements of complexity seemed to be the factors that determine the realized pattern of feeding linkages: heterogeneity in prey vulnerability (predator‐prey size ratios) and predator efficiency (vegetation cover for ambush predators, inaccessibility of epiphytic prey to bottom-feeding suckers). 5 . Because of the openness of the system, the community was able to respond to enrichment by a combination of demographic (production) and behavioural (migration) mechanisms. We argue that the food web model described an energetically favourable state, and that migration provided a ‘shortcut’ for the system to approach this state within a short growing season.

http://people.uleth.ca/~joseph.rasmussen/pdfs/DeBruyn-etal2004.pdf

Migration supports uneven consumer control in a sewage-enriched river food web

At Alexandra Fiord, Ellesmere Island, the diving beetles Hydroporus morio Aube and Hydroporus polaris Fall occur in a series of shallow ponds. Detailed habitat measurements of a temporary and a permanent pond revealed a more complex and extensive organic substrate and vegetation community, longer developmental time, and greater thermal budget in the permanent pond. Hydroporus polaris was most abundant in the temporary pond, but occurred in both; this species oviposited in the absence of macrophytic vegetation, completed larval development quickly, and pupated in the drying pond substrate, and adults dispersed in fall to moister overwintering sites. Hydroporus morio was restricted to the single permanent pond; this species took longer to complete larval development, pupated in wet moss, and overwintered as adults encased in ice on the vertical pond edge. We hypothesize that H. morio is excluded from temporary ponds in the arctic by its requirement for a relatively long development time. Alternatively, H. morio may require sheltered overwintering sites that temporary ponds do not offer.

https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0008347X00010014

Adrian M. H. deBruyn

Papers

Quantifying assimilation of sewage-derived organic matter by riverine benthos

The role of sewage in a large river food web

Altered body size distributions in a large river fish community enriched by sewage

Migration supports uneven consumer control in a sewage-enriched river food web

Comparative ecology of two species of hydroporus (coleoptera: dytiscidae) in a high arctic oasis