Vitamins are compounds with biological activity and they are essential and fundamental for normal health and organisms growth. Vitamins are available in diet and supplements and their deficiency can lead to some human diseases. The analysis of these compounds is indispensable for monitoring their content in pharmaceuticals and food in order to prevent some human diseases. Thin layer chromatography (TLC) is generally regarded as a common analytical method for screening, separation, and preliminary identification of compounds. TLC plays an important role in the quality control of food and drugs in order to investigate the ingredients and to detect the impurities and also for checking the purity and the stability of preparations.

Thin Layer Chromatography for the Analysis of Vitamins and Their Derivatives

Hydrophilic vitamins play an important role in human health The analysis of these compounds is indispensable for monitoring their content in pharmaceuticals and food in order to prevent some human diseases The separation and identification of eight hydrophilic vitamins, ie, B1, B2, B3, B5, B6, B9, B12, and C, was achieved by thin layer chromatography and Raman spectrometry After chromatographic experiments, the best results were obtained using programmed multiple development The unambiguous identification of superimposed spots can be done without using standards of vitamins

Separation and Identification of Eight Hydrophilic Vitamins Using a New TLC Method and Raman Spectroscopy

Micellar liquid chromatographic determination of nicotinic acid and nicotinamide after precolumn König reaction derivatization

Selected nicotinic acid derivatives have been chromatographed on RP-HPTLC plates with methanol-water in different volume proportions as mobile phases. Linear relationships were obtained between R M values of nicotinic acid derivatives and the volume fraction of methanol in the mobile phase. The retention values R M were extrapolated to zero methanol content and the lipophilicity values R M0 obtained were compared with measured (log P exp ) partition coefficients and values ( A log P s, IA log P , C log P , log P Kowin , and x log P ) calculated by use of five different software products. A linear relationship was obtained between R M0 and the slope, S , of the chromatography equation. The most significant correlation was found between the R M0 and A log P s values. For the nicotinic acid derivatives R M0 was a slightly better measure of lipophilicity than ϕ 0 . Chromatographic R M0 values can be use as a measure of the lipophilicity of the nicotinic acid derivatives investigated. Satisfactory linear corre...

Study of lipophilicity and application of selected topological indexes in QSAR analysis of nicotinic acid derivatives. Part I

Nicotinic acid (NAC) and its derivatives methyl nicotinate (MN), ethyl nicotinate (EN), isopropyl nicotinate (IPN), butyl nicotinate (BN), hexyl nicotinate (HN), benzyl nicotinate (BNN), nicotinamide (NAM), and N -methylnicotinamide (MNAM) have been investigated on RP18WF 254 HPTLC plates with methanol-water in different volume proportions as mobile phases. Linear relationships were obtained between R M values of the compounds and the volume fraction of methanol in the mobile phase. Retention values R M were extrapolated to zero methanol content and the lipophilicity values R M0(RP18WF254) obtained were compared with measured partition coefficients (log P exp ) and with partition coefficients ( A log P s, IA log P , C log P , log P Kowin , and x log P ) calculated using five different software products. The most significant correlation was found between R M0(RP18WF254) and A log P s values. The values of chromatographic lipophilicities R M0(RP18WF254) and ϕ 0(RP18WF254) indicate that lipophilicity of the ...

Study of lipophilicity and application of selected structural descriptors in QSAR analysis of nicotinic acid derivatives. Investigations on RP18WF254 plates. Part II

Separations of nicotinic acid and its derivatives on different stationary phases (silica gel, a mixture of silica gel and kieselguhr, polyamide 11, and RP-18) have been compared Relationships between R M values and mobile-phase composition were determined for ten nicotinic acid derivatives The results can be used to predict conditions suitable for extraction of the derivatives of nicotinic acid investigated

Agnieszka Szarkowicz

Papers

Use of TLC and RPTLC for separation of nicotinic acid derivatives