Showing papers by "Alan Davison published in 2005"
TL;DR: In vivo studies coupled with additional in vitro and ex vivo assessment show that 99mTc-1 has high and specific uptake in melanoma metastases in lungs and can potentially follow the temporal growth of these tumors.
Abstract: Evaluation of [99mTc]oxotechnetium(V) complexes of the amine-amide-dithiol (AADT) chelates containing tertiary amine substituents as small-molecule probes for the diagnostic imaging of metastatic melanoma has shown that technetium-99m–labeled AADT-(CH2)2-NEt2 (99mTc-1) has the highest tumor uptake and other favorable biological properties. We have, therefore, assessed this agent in a more realistic metastatic melanoma model in which, after i.v. tail injection, a highly invasive melanoma cell line, B16F10, forms pulmonary tumor nodules in normal C57BL6 mice. Small melanotic lesions develop in the lungs and, on histologic examination, appear as small black melanoma colonies, increasing in size and number with time after tumor cell injection. Groups of mice received tumor cell inocula of 2 × 105, 4 × 105, or 8 × 105 B16F10 cells; 14 days later, 2 hours after 99mTc-1 administration, lung uptake of 2.83 ± 0.21%, 3.63 ± 1.07%, and 4.92 ± 1.61% injected dose per gram of tissue (% ID/g), respectively, was observed, compared with normal lung uptake of 2.13 ± 0.2% ID/g ( P < 0.05). Additionally, a higher level of 99mTc-1 accumulation was seen 17 days after tumor cell inoculation as the lung lesions grew. These in vivo studies coupled with additional in vitro and ex vivo assessment show that 99mTc-1 has high and specific uptake in melanoma metastases in lungs and can potentially follow the temporal growth of these tumors.
29 citations
TL;DR: In this paper, the tetradentate umbrella ligand tris-(ortho-mercaptophenyl)phosphine (PS3 for the tri-anionic form) followed by various neutral donor ligands is the focus of this work.
9 citations
01 Jan 2005
TL;DR: In this article, the authors evaluated the performance of technetium-99m-labeled AADT (CH2)2-NEt2 ( 99m Tc-1) in lung metastatic melanoma.
Abstract: Evaluation of [ 99m Tc]oxotechnetium(V) complexes of the amine-amide-dithiol (AADT) chelates containing tertiary amine substituents as small-molecule probes for the diagnostic imaging of metastatic melanoma has shown that technetium-99m–labeled AADT-(CH2)2-NEt2 ( 99m Tc-1) has the highest tumor uptake and other favorable biological properties. We have, therefore, assessed this agent in a more realistic metastatic melanoma model in which, after i.v. tail injection, a highly invasive melanoma cell line, B16F10, forms pulmonary tumor nodules in normal C57BL6 mice. Small melanotic lesions develop in the lungs and, on histologic examination, appear as small black melanoma colonies, increasing in size and number with time after tumor cell injection. Groups of mice received tumor cell inocula of 2 10 5 ,4 10 5 ,o r 8 10 5 B16F10 cells; 14 days later, 2 hours after 99m Tc-1 administration, lung uptake of 2.83 F 0.21%, 3.63 F 1.07%, and 4.92 F 1.61% injected dose per gram of tissue (% ID/g), respectively, was observed, compared with normal lung uptake of 2.13 F 0.2% ID/g (P < 0.05). Additionally, a higher level of 99m Tc-1 accumulation was seen 17 days after tumor cell inoculation as the lung lesions grew. These in vivo studies coupled with additional in vitro and ex vivo assessment show that 99m Tc-1 has high and specific uptake in melanoma metastases in lungs and can potentially follow the temporal growth of these tumors. (Cancer Res 2005; 65(12): 4979-86)