Alessandra Basso

TL;DR: Covalently immobilised penicillin G acylase is active in both apolar and water miscible solvents and the porous siliceous matrix has been used also for the dehydration and storage of the enzyme in apolar media.

TL;DR: In this paper, it was shown that penicillin G amidase catalysed synthesis of ampicillin and cephalexin by enzymatic acyl transfer to the β-lactam antibiotic nuclei in a highly condensed system using mainly undissolved substrates, with no apparent aqueous liquid phase, was demonstrated.

TL;DR: It is demonstrated that the resin porosity greatly affects the efficiency in solid-phase biotransformations and that Synbead resins are valuable alternatives to swelling polymers for solid- phase chemistry and biocatalysis.

TL;DR: In this paper, the applicability of aerogels as solid supports for immobilisation of the enzymes PGA, thermolysin and α-chymotrypsin was assayed.

TL;DR: A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri of industrial applicability and the efficiency of the immobilized rPACP.rett was evaluated by studying the kinetically controlled synthesis of β‐lactam antibiotics (cephalexin), which is a crucial parameter for the feasibility of the process.