Mangiferin present in Curcuma amada was extracted with the help of microwave assisted extraction (MAE). The extraction solvent used was ethanol, which is eco-friendly and reduced the risk of environmental hazard. The mangiferin content was found to increase until 500 W, but decreased as the microwave power was increased further. A similar threshold was also obtained for microwave irradiation time. Following a mathematical analysis, an optimal mangiferin yield of 41 μg/mL was obtained from an extraction time of 15.32 s for a microwave power of 500 W.

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Microwave assisted extraction of mangiferin from Curcuma amada.

Mangiferin, a bioactive metabolite having potent antioxidant and pharmacological properties has been obtained in this work by microwave assisted extraction (MAE) technique, from Mangifera indica leaves. A Box–Behnken design was used to evaluate the effect of three parameters: microwave power, the time of extraction, and the ratio of solvent to raw material (mL/g) over the mangiferin yield. Irradiation time was the most important parameter on the recovery of mangiferin. The results indicated that the optimal conditions should be as follows: power of microwave 900 W, extraction time of 5 min and a solvent volume / vegetal material relation of 10. With these parameters the maximum mangiferin yield was 63.22%. The MAE process was compared with the conventional extraction in stirred tank. No significant statistical differences were found (p= 0.5639) between both extraction methods of mangiferin. The presence of the metabolite was conﬁrmed using high performance liquid chromatography. The method was validated with the guidelines set on the International Conference on Harmonization (ICH), for the validation of analytical procedures.

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Extraction of mangiferin from Mangifera indica L. leaves using microwave- assisted technique

A method for enhancing at least one of alertness, attention, concentration and memory in a person in need thereof, comprising co-administering an effective amount of mangiferin, norathyriol, or an extract comprising mangiferin or norathyriol; and caffeine to the person. The components may be co-administered in a single composition, or administered in separate compositions. The mangiferin and/or norathyriol exhibit synergistic effects on brain wave activity when co-administered with caffeine.

Compositions for enhancing brain activity

The invention discloses a crystal form V, a preparation method, a composition and use of a mangiferin compound (with a chemical name of 2-C-beta-D-glucopyranosyl-1,3,6,7-tetrahydroxyxanthone and an English name of mangiferin) and particularly discloses a crystal form-V solid matter state form of mangiferin in a solid state, a preparation method of a crystal form-V solid matter sample and application of a mangiferin crystal form-V matter as a pharmaceutical active component in the preparation various drugs for protecting livers and the gallbladder, relieving cough, eliminating phlegm, resistingcancers and hyperuricemia, preventing and treating gout, metabolic diseases of gout and central nervous system diseases. The molecular structural formula of mangiferin is as shown in the specification.

Mangiferin crystal form-VI matter as well as preparation method, composition and use thereof

The invention discloses a method for extracting mangiferin from mango leaves. The method includes the following steps: raw material processing, high-pressure extraction, aqueous two-phase extraction, desalination, and recrystallization to obtain the mangiferin. The method provided by the invention has the advantages of high mangiferin content, high extraction rate, simple process, suitability for production, low production cost and the like.

Method for extracting mangiferin from mango leaves

A process for preparing mangiferin from plants of the genus of Hedysarum (Hedysarum alpinum L., Hedysarum flavescens Rgl. et Schmalh) comprising extraction of xanthone glycosides from the aerial portion of the plants by means of a mixture acetone-water at a volume ratio of the mixture components of 1:0.5-2 respectively; the resulting extract is evaporated, the evaporated extract is filtered. To the filtered evaporated extract sulphuric acid is added to a pH of from 2 to 4, heated at reflux and filtered. The thus-prepared extract is treated with a non-polar organic solvent with the formation of two layers, wherefrom the upper aqueous layer contains xanthone glycosides. This upper layer is treated with butanol with the formation of two layers, wherefrom the upper layer comprises a solution of xanthone glycosides in butanol. This upper butanolic layer is evaporated. The precipitated mangiferin is separated and recrystallized.

Alla Andreevna Ananieva

Papers

Process for preparing mangiferin