Allen Nguyen

TL;DR: This work demonstrates three significant advantages of the Motorola CodeLink platform: sensitivity of one copy per cell, coefficients of variation in the hybridization signals across slides and across target preparations, and specificity in distinguishing highly homologous sequences.

TL;DR: It is demonstrated that a three-dimensional surface may enable use of shorter oligodeoxyribonucleotide probes and that hybridization time may be critical in improving the accuracy of microarray data.

TL;DR: RNA mixing experiments show a linear and quantitative amplification in probe hybridization signals for >6000 genes across the entire signal range, and the ability to discern biological and production variability through the use of different lots of commercial samples as visualized by hierarchical clustering is shown.

TL;DR: Incorporation of 2-aminoadenosine triphosphate into cRNA targets is a promising method for increasing signal detection in microarrays and can be optimized to minimize impact on yield of amplified material and to increase the number of expression changes that can be detected.

TL;DR: The oligonucleotide arrays and methods employed in this study can be used with cell lines, tissue sections, blood, and other fluids, and show high reproducibility and should allow an investigator to discern biological and regional variability from differential expression.