André de Villiers

TL;DR: It is found that UPLC conditions hold advantages in terms of speed of analysis, for required theoretical plate counts up to approximately 80,000 and practical maximum efficiency.

TL;DR: The effects of viscous heat dissipation on some important HPLC parameters, such as efficiency and retention factors, using 2.1mm columns at pressures up to 1000 bar have been investigated from both a theoretical and experimental point of view.

TL;DR: In this paper, an overview of the latest developments and their application in flavonoid analysis since 2009 is provided, focusing on methodologies which provide improved LC separation of flavonoids in terms of speed and/or resolution, including ultra high pressure LC (UHPLC), monolithic and superficially porous phases, high temperature LC (HTLC) and comprehensive two-dimensional LC (LC×LC).

TL;DR: This review seeks to provide an updated overview of the application of recent advances in HPLC to phenolic separation, with the emphasis on how these methodologies can contribute to improve performance in HP LC analysis of phenolics.

TL;DR: Among the phenolic compounds, scolymoside, hesperidin and iriflophenone-3-C-β-glucoside contents were the most variable, and a combination of the measured parameters could be useful in product standardization by providing a basis for specifying minimum levels.