Immunosensors--principles and applications to clinical chemistry.

Recent developments in the field of screen-printed electrodes and their related applications.

Fatty acid-binding proteins as plasma markers of tissue injury

Intestinal-type and liver-type fatty acid-binding protein in the intestine. Tissue distribution and clinical utility

This review summarizes scientific research activity on biosensors, especially screen-printed, electrode-based biosensors. The basic configurations of biosensors based on screen-printing technology are discussed and different procedures for immobilization of the biorecognition component are reviewed. Theoretical aspects are exemplified by practical environmental and food-analysis applications of screen-printed, electrode-based biosensors.

Biosensors based on screen-printing technology, and their applications in environmental and food analysis

An immunosensor based on disposable electrodes for rapid estimation of fatty acid-binding protein, an early marker of myocardial infarction.

Cytoplasmic heart-type fatty acid-binding protein has recently gained much attention in clinical diagnosis as a very early marker of acute myocardial infarction. Immunoassays have been developed for determination of this protein in plasma and urine samples. In the present study it is shown that those types of fatty acid-binding proteins which are abundant in tissues other than heart and muscle do not interfere with immunochemical determination of heart-type fatty acid-binding protein. To provide sufficient protein of consistent quality as standard in these immunoassays, human heart-type fatty acid-binding protein was cloned, expressed in Escherichia coli and purified to homogeneity. For quantitation of the recombinant protein its extinction coefficient was determined. Comparison of the recombinant and tissue-derived proteins by a variety of methods revealed both proteins to show similar kinetic as well as equilibrium constants with respect to two monoclonal antibodies currently applied in immunochemical detection of heart-type fatty acid-binding protein. Both preparations were indistiguishable in sandwich-ELISA and immunosensor measurements. A high stability of the recombinant protein was proven by ELISA measurements during storage and several freeze and thaw cycles. Thus, recombinant and tissue-derived heart-type fatty acid-binding proteins are immunochemically equivalent. The recombinant human heart-type fatty acid-binding protein is now available as standard for immunoassays.

Andrea Schreiber

Papers

An immunosensor based on disposable electrodes for rapid estimation of fatty acid-binding protein, an early marker of myocardial infarction.

Recombinant human heart-type fatty acid-binding protein as standard in immunochemical assays.

Multicenter evaluation of an amperometric immunosensor for plasma fatty acid-binding protein: an early marker for acute myocardial infarction.