Showing papers by "Andrew C. Issekutz published in 1981"

The in vivo quantitation and kinetics of monocyte migration into acute inflammatory tissue.

Abstract: Mononuclear leukocytes isolated from the blood of rabbits, were labeled with 51Cr and returned to the animal intravenously. 51Cr-labeled monocytes disappeared from the blood with a half-life of 39.0 +/- 2.51 hours. Numerous acute inflammatory lesions were produced by the intradermal injection of Escherichia coli into the skin of the back of a rabbit. The animal was sacrificed after 1 hour, and the radioactivity in each lesion was determined. Monocyte accumulation was substantial by the time a lesion was 1 hour old. The maximum rate of accumulation occurred at 3--4 hours, and monocytes continued to enter the lesions at 25% of the maximal rate for at least 24 hours. Monocytes initially migrate into bacterial inflammatory sites simultaneously with neutrophils and histologically become the predominant cell type after 12 hours because they continue to migrate into these lesions long after neutrophils have stopped. The kinetics of monocyte migration is related to other aspects of inflammation.

991 the use of plasma infusion for neonatal sepsis

Abstract: To assess the value of fresh frozen plasma (FFP) infusion to neonates with sepsis we obtained blood samples from 20 infants with suspected sepsis before and after i.v. FFP infusion (20ml/kg). Classical (CCP) and alternative (ACP) complement hemolytic activities, opsonic activity (OA) against the infecting organism and coagulation factors VIII and XIII activity and antigen were evaluated. Sera from mothers of septic infants, from matched control infants, and from recalcified FFP were compared to adult pooled serum. Of the 20 study infants, 7 had sterile blood and CSF cultures while 13 had isolates from blood, CSF or middle ear, including 7 group B streptococcus and 2 L. monocytogenes. Low CCP and/or ACP activity, present in half of the patients was corrected in all after plasma infusion (p< .001). ACP and CCP activities were normal in FFP. Depressed OA to the infecting organism (p< 0.01 vs control infants) was seen before FFP. After FFP, OA improved (7/12) or was unchanged in all but 1 culture +ve infant. Compared to adult serum, OA was similar in control infants (median 109%) and in FFP (104%) but lower in mothers of infected infants (71%). Increased ratios of Factor VIII and XIII antigen/activity compatible with proteolysis and/or mild DIC were present prior to FFP and approached normal 20 hours later. We conclude that FFP infusion to septic infants is safe. The observed changes in complement and opsonic activity may benefit the depressed humoral host defense system. (MRC grant No. 210)