Nitric Oxide Donors: Chemical Activities and Biological Applications

Nitric oxide and mitochondrial respiration.

Non-heme iron nitrosyls in biology

NO, nitrosonium ions, nitroxide ions, nitrosothiols and iron-nitrosyls in biology : a chemist's perspective

Nitric oxide (NO) has diverse roles in intercellular communication and (at higher levels) in immune-mediated cell killing. NO reacts with many cellular targets, with cell-killing effects correlated to inactivation of key enzymes through nitrosylation of their iron-sulfur centers. SoxR protein, a redox-sensitive transcription activator dependent on the oxidation state of its binuclear iron-sulfur ([2Fe-2S]) centers, is also activated in Escherichia coli on exposure to macrophage-generated NO. We show here that SoxR activation by NO occurs through direct modification of the [2Fe-2S] centers to form protein-bound dinitrosyl-iron-dithiol adducts, which we have observed both in intact bacterial cells and in purified SoxR after NO treatment. Functional activation through nitrosylation of iron-sulfur centers contrasts with the inactivation typically caused by this modification. Purified, nitrosylated SoxR has transcriptional activity similar to that of oxidized SoxR and is relatively stable. In contrast, nitrosylated SoxR is short-lived in intact cells, indicative of mechanisms that actively dispose of nitrosylated iron-sulfur centers.

https://www.pnas.org/content/pnas/97/10/5146.full.pdf

Direct nitric oxide signal transduction via nitrosylation of iron-sulfur centers in the SoxR transcription activator

Formation of paramagnetic mononuclear iron nitrosyl complexes from diamagnetic di- and tetranuclear iron-sulphur nitrosyls: characterisation by EPR spectroscopy and study of thiolate and nitrosyl ligand exchange reactions

Nitrogen-15 and carbon-13 NMR study of Roussin salts and esters and of pentacyanoferrate complexes

Ligand exchange processes in some iron-sulphur-carbonyl and -nitrosyl complexes

Nitrosylation of 2Fe-2S and 4Fe-4S models for iron-sulphur redox proteins

Homonuclear spin-spin coupling through iron in simple inorganic ions

Andrew R. Hyde

Papers

Formation of paramagnetic mononuclear iron nitrosyl complexes from diamagnetic di- and tetranuclear iron-sulphur nitrosyls: characterisation by EPR spectroscopy and study of thiolate and nitrosyl ligand exchange reactions

Nitrogen-15 and carbon-13 NMR study of Roussin salts and esters and of pentacyanoferrate complexes

Ligand exchange processes in some iron-sulphur-carbonyl and -nitrosyl complexes

Nitrosylation of 2Fe-2S and 4Fe-4S models for iron-sulphur redox proteins

Homonuclear spin-spin coupling through iron in simple inorganic ions