Leukaemia specific antigens have been isolated from the membranes of dinitrophenylated normal human leukocytes (104 molecules of l-fluoro, -2, 4-dinitrobenzene per cell). Affinity chromatography on antibody-sepharose column separated two macromolecular moieties with molecular weights of 21000 and 48000. The isolated antigens elicited immune response specific against human leukaemic cells. The use of oil-in-water emulsion with isolated antigens was essential during immunization to achieve appropriate hydrophylic-hydrophobic conformation similar to that seen on the native cell surfaces.

Anjali A Karande

Papers

Isolation of leukaemia specific antigenic macromolecular moiety from dinitrophenylated normal human leukocytes