Showing papers by "Anne K. Vidaver published in 1974"
TL;DR: A new species of Corynebacterium isolated from field corn is described, characterized by orange-pig-mented colonies, inability to grow on 0.005% triphenyltetrazolium chloride agar, specific bacteriophage sensitivity, and a guanine plus cytosine content of 73.5 mol%.
Abstract: A new species of Corynebacterium isolated from field corn is described. The isolates form a homogeneous group that is recognized as a new species, Corynebacterium nebraskense. The organism is characterized by orange-pig-mented colonies, inability to grow on 0.005% triphenyltetrazolium chloride agar, specific bacteriophage sensitivity, and a guanine plus cytosine content of 73.5 mol%. These and other characters differentiate this organism from other described phytopathogenic corynebacteria. The type strain of C. nebraskense is Fur-1 (= ATCC 27822 = NCPPB 2578).
58 citations
TL;DR: Evidence is presented which suggests that the dsRNAs may contain a short single-stranded RNA tail, and the two smaller RNA segments reanneal rapidly even at low temperatures; in contrast, the large dsRNA reannealed only at higher temperatures.
Abstract: The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage φ6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of the large dsRNA segment. The two smaller RNA segments reanneal rapidly even at low temperatures; in contrast, the large dsRNA reannealed only at higher temperatures. Evidence is also presented which suggests that the dsRNAs may contain a short single-stranded RNA tail.
42 citations
TL;DR: The syringacin was heat and alcohol sensitive, but resistant to trypsin, chymotrypsin and carboxypeptidase, Pronase, protease, lysozyme, steapsin, deoxyribonuclease, and ribonucleasing.
Abstract: Syringacin 4-A, a bacteriocin produced by Pseudomonas syrinagae 4-A, was obtained by induction with ultraviolet irradiation or mitomycin C. Approximately 1,000-fold purification of the bacteriocin was achieved by manganous chloride precipitation, differential centrifugation, and chromatography on hydroxyapatite columns. The purified syngacin was homogeneous on hydroxyapatite columns and sucrose density gradients; it also sedimented as a single entity in the analytical ultracentrifuge. The buoyant density of purified syringacin in cesium chloride was 1.294 g/ml. The sedimentation coefficient was calculated as 120 S , and the diffusion coefficient was 6.49 × 10 −8 cm 2 /s. The molecular weight was calculated as 1.6 × 10 7 from physical data and 1.7 × 10 7 from biological data. The syringacin was composed of about 88.4% protein, 8.5% arabinose, 2.2% galacturonic acid, and 0.7% glucosamine. Amino acid analysis indicated a predominance of leucine (12.1%), aspartic acid (12.2%), and glutamic acid (12.7%). The ultraviolet spectrum showed a maximum absorbance peak at 276 nm. The syringacin was heat and alcohol sensitive, but resistant to trypsin, chymotrypsin, carboxypeptidase, Pronase, protease, lysozyme, steapsin, deoxyribonuclease, and ribonuclease. Maximum pH stability was between 5 and 8. Crude bacteriocin was stable at room temperature for at least a year, and purified material was stable for at least 3 months at 4 C. Images
25 citations
TL;DR: The interferon-inducing capabilities of the three molecular segments of bacteriophage phi6 double-stranded ribonucleic acid increased with increasing molecular weight.
Abstract: The interferon-inducing capabilities of the three molecular segments of bacteriophage φ6 double-stranded ribonucleic acid increased with increasing molecular weight.
3 citations