Showing papers by "Aoife Morrin published in 2005"
TL;DR: In this paper, a novel aqueous-based nanoparticulate polyaniline (PANI), doped with dodecylbenzenesulfonic acid (DBSA), was demonstrated for H2O2 sensing.
Abstract: The biosensor described here uses a novel aqueous-based nanoparticulate polyaniline (PANI), doped with dodecylbenzenesulfonic acid (DBSA). The nanoparticles were applied to a glassy carbon electrode surface by electrodeposition techniques, and horseradish peroxidase (HRP) was subsequently electrostatically adsorbed to the nanoparticle-modified surface. This biosensor format was demonstrated for H2O2 sensing. Electrodeposited polymer nanoparticles resulted in highly ordered conductive nanostructured films, which were examined by scanning electron microscopy (SEM), atomic force microscopy (AFM), profilometry and spectroelectrochemistry. The surface of the films were characterized by a uniform array of nanoparticulate PANI (nanoPANI/DBSA) nodules and were shown to have a thickness of 350 nm. Physical techniques have shown that the nanofilms possess properties which allow for uniform electrostatic adsorption of protein to take place. This effective biosensor format, exhibits higher signal-to-background ratios and shorter response times than previous PANI biosensor configurations.
98 citations
TL;DR: The feasibility of casting enzyme with polyaniline nanoparticles is demonstrated in this paper, where the nanoparticles were simply cast by a drop-coating method onto the surface, after suitable pH adjustment, it was shown that horseradish peroxidase (HRP) enzyme could be added to the dispersion, and cast simultaneously with the conducting polyaninine.
83 citations
TL;DR: In this paper, the properties of poly (2methoxyaniline-5-sulfonic acid) (PMAS), a water-soluble polymer, has been investigated as a potential mediator for biosensors.
40 citations
22 Jun 2005
TL;DR: In this article, a polyaniline-based amperometric immunosensor platform has been developed over several years in the NCSR at Dublin City University, which is achieved by electropolymerisation of polyvinylsulphonate-doped PANI/PVS onto the surface of a disposable screen-printed carbon-paste electrode.
Abstract: This effective polyaniline-based amperometric immunosensor platform has been developed over several years in the NCSR at Dublin City University. Construction of this sensor is achieved by electropolymerisation of polyvinylsulphonate-doped polyaniline (PANI/PVS) onto the surface of a disposable screen-printed carbon-paste electrode. Antibodies are then doped onto the surface of the polymer to yield an immunosensor capable of rapid, single-step, separation-free assays for real-time monitoring. Although scanning electron microscopy (SEM) can provide adequate surface information as to the overall topography of protein-modified PANI/PVS films, the resolution of individual proteins or groups of proteins was not possible. However, through the use of proteins conjugated to non-fading, electron-dense particles such as gold, it was possible to indirectly visualise the distribution of proteins. A colloidal gold-labelled anti-goat antibody (Ab) was used for the visualisation of two immunosensor platforms where both anti-atrazine single chain Ab and anti-biotin Ab were immobilised on the polymer surface. Various incubation and washing steps were carried out to allow specific interactions between the immobilised Ab and anti-goat-gold Ab to occur. A silver enhancement treatment was optimised in order to improve the visualisation of the gold label. The silver enhancement caused the reduction of silver ions, resulting in the precipitation of metallic silver around the gold colloids, so as to enlarge the gold particles for enhanced visualisation. Energy Dispersive X-ray (EDX) analysis was also performed to assess the amount of silver on the surface. Characterisation Of An Amperometric Immunosensor Surface By Scanning Electron Microscopy
1 citations