Showing papers by "Archana Sharma published in 1995"
TL;DR: In this paper, the anticlastogenic activities of a crude extract of leaves of spinach-beet (Beta vulgaris var. benghalensis Hort.) and equivalent amounts of chlorophyll extracted from the leaves and of synthetic CH in reducing cytotoxicity were compared following exposure of mice in vivo to a known clastogen chromium (VI) oxide.
Abstract: The anticlastogenic activities of a crude extract of leaves of spinach-beet (Beta vulgaris var. benghalensis Hort.) and equivalent amounts of chlorophyll extracted from the leaves and of synthetic chlorophyllin in reducing cytotoxicity were compared following exposure of mice in vivo to a known clastogen chromium (VI) oxide. Male Swiss albino mice were administered orally the vegetable extract for 7 consecutive days and then exposed to the clastogen by gavage (20 mg/kg b wt). For comparison, equivalent amounts of extracted chlorophyll and synthetic chlorophyllin were administered to the mice, 2 h before exposure to the same dose of the metal. Chromosomes were studied from bone marrow cells 24 h after exposure, following colchicine-hypotonic-fixative-flame drying-Giemsa staining schedule. Chlorophyllin and the crude extract, when given alone, did not induce chromosomal aberrations and reduced the clastogenic effects induced by chromium (VI) oxide to a statistically significant level, indicating a protective action. Chlorophyll, however, produced a significant increase of chromosomal aberrations compared with control, when administered alone and was not able to reduce the clastogenicity of the metallic salt to a significant level.
16 citations
TL;DR: The present study was undertaken on groups of workers in metal industries, exposed directly and indirectly to complex environmental work conditions, using chromosomal aberrations as the endpoint for monitoring.
Abstract: The need for determining the potential genotoxic effects of longterm exposure to complex workplace environment has been widely accepted (see IARC 1984, ICPEMC committee 1985, ICEM 1989) and a number of techniques have been adopted for the purpose (for review see Anderson 1988). Screening for chromosomal aberrations in cultured lymphocytes is now an accepted method for monitoring populations exposed to various chemicals, singly or in mixtures (Forni 1983, Galloway et al. 1986, Sorsa et al. 1982, 1983). Such studies are of particular importance in India, where industrialisation has progressed rapidly in certain belts during the past four decades. The present study was undertaken on groups of workers in metal industries, exposed directly and indirectly to complex environmental work conditions, using chromosomal aberrations as the endpoint for monitoring. Individuals were matched with respect to age, sex, nutritional status and addiction.