Quantification of immobilized Candida antarctica lipase B (CALB) using ICP-AES combined with Bradford method.

Four types of chitosan bead (CB) were tested to find the removal efficiency for cation and anion from solution. Hydrogel chitosan bead (HCB) was first prepared and then modified by chemical and physical treatment. Both the Cu(II) and phosphate removal were investigated based on the batch removal efficiency, a pH test, kinetics, and an isotherm test. For Cu(II) application, the highest Cu(II) uptake (approximately 83%) was obtained for the HCB; the modification by crosslinking by glutaraldehyde (GA) and air drying decreased this to approximately 60%, while phosphate removal was not affected by crosslinking and drying. Below pH 4.0, the removal efficiency of Cu(II) was decreased by approximately 20% for all type of CB, while phosphate uptake was determined by the type of CB, regardless of the initial pH. The maximum Cu(II) and phosphate uptake (Q) were 129 and 232 mg/g for the HCB and dried chitosan bead (DCB), respectively. The phosphate sorption rate was faster than Cu(II) at the beginning of the reaction for the hydrogel bead, while the dried bead reached the equilibrium in 72 h.

An Experimental Application of Four Types of Chitosan Bead for Removal of Cationic and Anionic Pollutants

Synthesis and Characterization of Chitosan-Glutaraldehyde Sorbent Materials For the Removal of Arsenate

Metabolic regulation mechanism of Trametes gibbosa CB_1 on lignin.

Coomassie Brilliant Blue G-250 (CBBG) was bound to the reaction products of glutaraldehyde with various compounds containing amino group. The reaction products of acetaldehyde and malonaldehyde with the amines did not bind with CBBG, indicating that the binding was specific for the product of glutaraldehyde. The dye binding products rapidly formed in alkaline pH. Gel filtration chromatography indicated that a reaction product of more than 2 kDa has a dye- binding ability. The reaction product of glutaraldehyde with 6-aminohexanoic acid was separated with SP-Sephadex. CBBG was bound to the first eluted fraction from the column with highest affinity. The absorbance at 595nm by CBBG binding was linearly related to this lyophilized fraction at a concentration of up to 250μg/ml, suggesting its applicability to the determination of the reaction product of glutaraldehyde and amine. The present method should contribute to our understanding of the chemistry of glutaraldehyde associated with diverse fields, such as enzyme technology and biochemistry.

https://www.jstage.jst.go.jp/article/analsci1985/13/1/13_1_5/_pdf

Aya Sorita

Papers

Determination of the Reaction Product of Glutaraldehyde and Amine Based on the Binding Ability of Coomassie Brilliant Blue