The number of angiosperm species for which nuclear DNA amount estimates have been made has nearly trebled since the last collected lists of such values were published, and therefore, publication of a more comprehensive list is overdue. This paper lists absolute nuclear DNA amounts for 753 angiosperm species. The data were assembled primarily for reference purposes, and so the species are listed in alphabetical order, as this was felt to be more helpful to cyto- and biochemists whom, it is anticipated, will be among its major users. The paper also reviews aspects of the history, nomenclature, methods, accuracy and problems of nuclear DNA estimation in angiosperms. No attempt is made to reconsider those aspects of nuclear DNA estimation which have been fully revised previously, although the bibliography of such aspects is given. Instead, the paper is intended as a source of basic information regarding the terminology, practice and limitations of nuclear DNA estimation, especially by Feulgen microdensitometry, as currently practiced.

Nuclear DNA Amounts in Angiosperms

The role of polyploidy per se in the development of evolutionary novelty remains one of the outstanding questions in flowering plant evolution. Since chromosome doubling usually is associated with hybridization, the effects of doubling are difficult to uncouple from those of hybridity and recombination. Synthetic polyploids in crops typically are inferior to their diploid counterparts under conditions to which the diploids are adapted. This observation has suggested to many that chromosome doubling is a hindrance to progressive evolution. Evidence is presented herein from biochemical, physiological, developmental, and genetical sources which indicates that the nucleotypic effects of chromosome doubling are not necessarily negative. Indeed chromosome doubling may "propel" a population in to a new adaptive sphere, and render it capable of occupying habitats beyond the limits of its diploid progenitor. This postulate is consistent with what we know of the ecological tolerances of diploids and related polyplo...

Polyploidy and Novelty in Flowering Plants

All else being equal, polyploids are expected to have larger C-values (amount of DNA in the unreplicated gametic nucleus) than their diploid progenitors, increasing in direct proportion with ploidy. This expectation is observed in some polyploid series, especially those newly formed, but there are examples suggesting that C-values in particular polyploids are less than expected. The availability of the Angiosperm DNA C-values database (http:// www.rbgkew.org.uk/cvavhomepage.html) has allowed this question to be addressed across a broad range of angiosperms and has revealed striking results deviating from expectation: (i) mean 1C DNA amount did not increase in direct proportion with ploidy, and (ii) mean DNA amount per basic genome (calculated by dividing the 2C value by ploidy) tended to decrease with increasing ploidy. These results suggest that loss of DNA following polyploid formation, or genome downsizing, may be a widespread phenomenon of considerable biological significance. Recent advances in our understanding of the molecular events that take place following polyploid formation together with new data on how DNA amounts can both increase and decrease provide some insights into how genome downsizing may take place. The nature of the evolutionary forces that may be driving DNA loss are also discussed.

Genome downsizing in polyploid plants

A theory of seed plant invasiveness: The first sketch

[A & B chromosomes. I].

Chromosome numbers have been determined and karyotypes studied in several species of the genus Briza L. Nuclear DNA amounts have also been determined for some of the species and show a considerable variation between the species. The evolution of the karyotypes, particularly that of B. minor, is discussed and an attempt made to relate nuclear DNA content to karyotypes, breeding systems and habit in the different species.

B. G. Murray

Papers

The cytology of the genus Briza L. ( Gramineae )