Gene manipulation in plant improvement , Gene manipulation in plant improvement , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی

Gene manipulation in plant improvement

Role of biotechnological interventions in the improvement of castor (Ricinus communis L.) and Jatropha curcas L.

Somatic embryogenesis and plant regeneration from cotyledons of walnut, Juglans regia L.

Techniques for the regeneration of Jatropha curcas L. from various explants have been developed. Regeneration from hypocotyl, petiole and leaf explants was evaluated on a range of concentrations of zeatin, kinetin and N6 benzyladenine (BA) either singly or in combination with indole-3-butyric acid (IBA). Higher regeneration from hypocotyl and petiole explants was obtained on BA with IBA than on zeatin- or kinetin-supplemented media. Leaf discs from the third expanding leaf exhibited higher regeneration potential than those from the fourth leaf. Independent of the explant type, direct adventitious shoot bud induction was recorded highest on MS medium with 2.22 μM BA and 4.9 μM IBA. Although the same BA concentration but with reduced IBA concentration (0.49 μM) proved effective in callus mediated regeneration from hypocotyl and leaf explants, the petioles required lower concentrations of the two growth regulators (0.44 μM BA and 0.49 μM IBA). Regenerated shoots could be rooted on growth regulator-free gelled full-strength MS medium. Following simple hardening procedures, the in vitro-raised plants could be transferred to soil and grown to maturity in the field.

Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas

The storage-protein content of non-zygotic and zygotic embryos of B. napus was compared, using antibodies to guantitate 12S storage protein in extracts by rocket immunoelectrophoresis. Non-zygotic embryos were induced from microspores in anther culture and on the hypocotyls of zygotic embryos in culture. All embryo-like structures were found to contain 12S storage protein, whereas preculture anthers, anthers from which embryos had been removed, and regenerated shoots did not have detectable 12S storage protein. In zygotic embryos, 12S storage protein was first detected at the cotyledon stage, but microsporic embryos contained storage protein at the globular and heart stages. Storage protein levels in microsporic and hypocotyl embryos were low relative to those in zygotic embryos. The largest microsporic embryo had a storage protein concentration of 13 μg mg-1 fresh weight, almost 10 times lower than a mature zygotic embryo. Thus, although storage proteins are present in both zygotic and non-zygotic embryos, the timing and extent of accumulation differ.

https://www.jstor.org/stable/pdfplus/23376230.pdf

Non-zygotic embryos of Brassica napus L. contain embryo-specific storage proteins.

Morphogenesis in endosperm cultures

Isolation of sodium chloride-tolerant plantlets of Kickxia ramosissima under in vitro conditions

Chilling induces development of embryoids into plantlets in Eschscholzia

Thein vitro embryoids ofEschscholzia californica do not form normal plantlets in cultures However, 6-benzylaminopurine and gibberellic acid, when added to the medium, partially alleviate this inhibition Experiments involving decotylization, and culture of embryoids at different stages, indicated the cardinal role of cotyledons in plumular growth

B.M. Johri

Papers

Morphogenesis in endosperm cultures

Isolation of sodium chloride-tolerant plantlets of Kickxia ramosissima under in vitro conditions

Chilling induces development of embryoids into plantlets in Eschscholzia

In vitro Responses of Embryoids ofEschscholzia californica

Response of Crown Gall Tissue to a Morphactin