Restorative dental materials

AIM::To compare the cytotoxicity of three kinds of DBA(PrimeBondNT,Pb;XenoⅢ,Xe;AdheSE,Ad)to the dental pulp cells in vitro.METHODS:Dental pulp cells were cultured in vitro.Three kinds of DBA(5 μl) were eluted with cured medium for 48 and 24 hours.Cytotoxicity was determined by using MTT assay.RESULTS: Following 24-hour exposure of cells to DBA cured for 10 or 40 seconds,pulp cells became rounder,rounded in appearance and lost their normal organization.By ANOVA and Dunnett-t test all materials were significantly cytotoxic compared with controls(P0.01).Pb was the least toxic sealer amongst the tested agents.Xe and Ad cured for 40 seconds lessened the cytotoxity compared with those cured for 10 seconds by student-t test(P0.01).CONCLUSION: DBA may exert some cytotoxic effects on the dental pulp,especially when DBA-curing is insufficiently complete.Sufficiently curing may lessen the DBA cytotoxity.[

Cytotoxicity of three dentin bonding agents on human dental pulp cells

The toxic potential of the endodontic sealers ingredients, especially the unreacted monomer, that can irritate the periapical tissue and interfere with the healing process, thus having a negative impact on the biocompatibility of the material. The aim of this study was to evaluate the influence of three experimental endodontic sealers on cells viability in vitro. Human umbilical vein endothelial cells (HUVEC) were used. The experiments were done with solid samples and extracts of sealers in artificial saliva and water. The experiments evaluated the cytotoxicity of the residual monomers that resulted from the tested composites. The decrease in cell viability was quantified by colorimetric measurement of formazan. The components of the sealers dissolved in artificial saliva and water were determined by high performance liquid chromatography (HPLC). The HUVEC are a novelty for testing the endodontic sealers biocompatibility, with certain advantages compared to other cell types used in the literature, e.g. HELA cells, fibroblasts. The data showed that cytotoxicity was directly linked with the unreacted monomer — 2-hydroxyethyl methacrylate (HEMA) present in these composites. Two of the three formulations had little or no cytotoxic effect, which makes them suitable for further testing in order to be used in endodontic treatment.

Baldea Ioana

Papers

Testing endodontic sealers on human umbilical vein endothelial cells