Showing papers by "Baojun Xu published in 2001"
Journal Article•
TL;DR: In this article, a method for the simultaneous and precise determination of lactone form and acid form monacolin K in red yeast rice by HPLC was developed, which is based on the reversed-phase column, and the acidified mobile phase consisting of acetonitrile : 0.1% trifluoroacetic acid (TFA) water soln : 62 :38.
Abstract: A method for the simultaneous and precise determination of lactone form and acid form monacolin K in red yeast rice by HPLC was developed in this study. The standard of acid form monacolin K was prepared by alkaline hydrolysis of its lactone form, which was purchased from Sigma company. The optimum HPLC system for the separation and quantification of acid form and lactone form monacolin K is based on the reversed-phase column, and the acidified mobile phase consisting of acetonitrile : 0.1% trifluoroacetic acid (TFA) water soln : 62 :38, the low limit detection amount was 5 ng (i.e.10 l injection of 0.5 /ml) . And the optimal extracting system for monacolins in red rice was also presented here
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