Bart Panis

TL;DR: The phenol extraction protocol was optimized for banana and was subsequently applied to two other plant species: apple and potato, and is useful for guiding the experimental setup of many other nonmodel species, containing various interfering elements.

TL;DR: It is demonstrated that a partitioning step into n- butanol during sample preparation is essential and that gradient HPLC analysis is necessary to prevent sample carryover between injections, and that the starting composition of the mobile phase must be sufficiently hydrophobic to allow direct injection of the n-butanol extracts without peak splitting, tailing, and other artifacts.

TL;DR: One millimeter sized shoot-tips excised from rooted in vitro plants of the genera Musa and Ensete were successfully cryopreserved with the droplet vitrification technique and ultra rapid freezing and thawing rates proved to be essential for high and reproducible post-thaw regeneration rates.

TL;DR: A simple protocol to allow the production of transgenic banana plants using accelerated particles coated with DNA revealed the stable integration of the transferred genes into the banana genome.

TL;DR: The power of 2DE for non-model plants in combination with multivariate data analysis and MS/MS identification and will evaluate possible alternatives are illustrated.