Showing papers by "Bernhard Tribukait published in 1981"

DNA pattern and cytological findings in fine-needle aspirates of untreated prostatic tumors. A flow-cytofluorometric study

Abstract: The cellular DNA content in fine-needle prostatic aspirates from 500 untreated patients was determined by flow cytofluorometry. According to the DNA patterns diploid, tetraploid, and non-tetraploid aneuploid cases were identified. In 301 cytologically benign cases more than 90% showed diploid DNA patterns. Among 166 carcinomas the incidence of aneuploid DNA values increased with the degree of anaplasia, ie, 44% in well-differentiated, 78% in moderately differentiated, and 97% in poorly differentiated tumors. In aneuploid cases of well-differentiated carcinomas almost exclusively tetraploid DNA patterns were observed, while in poorly differentiated carcinomas about 80% showed non-tetraploid aneuploid DNA distributions. Among aneuploid cases of moderately differentiated carcinomas 2/3 were tetraploid and 1/3 non-tetraploid aneuploid. Morphologically similar tumors may thus be separated by the DNA profiles. The biological significance of these results must be further evaluated by clinical follow-up of the patients.

The Clinical Implications of DNA Characteristics in Human Pituitary Tumour Disease

Abstract: DNA analysis was performed in 29 cases of various endocrinological types of human pituitary tumours. The degrees of ploidy and proliferation, expressed as the proportion of cells in S phase, were determined. In 12 cases (41%) aneuploidy occurred. The proportion of aneuploid cells in these tumours was very high, in general around 95% measured. The aneuploid group of pituitary tumours showed a large variation of the percentage of cells in S-phase (range: 3.3–13.7%; mean: 8.3±5.3) in contrast to diploid tumours which showed a considerably smaller variation (range: 3.4–8.8 %; mean: 5.5±2.1). Tatients with aneuploid tumours had a mean age at operation of 42.8±11.1 years in contrast to 51.5±13.8 years for patients with diploid tumours. Aneuploidy occurred more frequently in tumours causing acromegaly with a concomitant secretion of GH and PRL than in other endocrinological types of tumours. Patients with aneuploid tumours had a mean case history of 2.9±1.9 years, as compared to 10.5±4.9 years for patients with ...

Cellular DNA Content of Pituitary Tumours: Its Relationship to Morphology and Hormonal Type of Tumour

Abstract: The DNA content of 24 human pituitary tu- mours was analysed with flow-cytofluorometry, and com- pared with the morphologic characteristics of the tumours. Fifteen tumours had a diploid and 9 had an aneuploid DNA pattern. Minimal and moderate degrees of mitosis and nuclear polymorphism can possibly occur more fre- quently in aneuploid pituitary tumours than in diploid pituitary tumours (mitoses: 56% versus 27 %; nuclear polymorphism: 78 % versus 53 %). Otherwise no correla- tion between the degree of ploidy and the histopathologic or ultrastructural features could be registered. Thus, pitui- tary tumours of the same morphology can be subdivided according to their ploidy pattern.

DNA Characteristics of Human Pituitary Tumours

Abstract: Biopsies from pituitary tumours in 8 patients were analysed with DNA flow-cytofluorometry immediately after surgery and after organ culture. Good agreement was found between the degrees of ploidy and of the relative proportions of cells in different phases of the cell cycle, indicating a good stability and reproducibility of pituitary tumour explants in vitro. Analysis of DNA content indicated a homogeneous cell population in tumours before and after explantation. No apparent correlation was observed between hormone secretion rate in diploid and aneuploid tumours, respectively, and DNA characteristics.

Aneuploid DNA pattern in human pituitary adenomas

Abstract: The DNA contents of 29 histologically and ultrastructurally benign pituitary tumours were analyzed with flow-cytofluorometry. — Cytogenetic malignancy, i.e., aneuploidy, was found in 12 cases, independent of endocrinological type of tumour. In 17 cases a diploid DNA content occurred. In aneuploid tumours varying percentage of cells in S-phase was found indicating different growth patterns. Aneuploid DNA pattern was often found in young patients with a short case history. Our findings indicate that analysis of cellular DNA content may reflect the biological properties of pituitary tumours, independent of morphologic characteristics and type of hormone secretion.