An approach to hypothermic renal preservation.

Successful 96- and 144-hour experimental kidney preservation: a combination of standard machine preservation and newly developed normothermic ex vivo perfusion.

• Nonpulsatile left ventricular (LV) bypass was achieved in nine alert, active calves for periods of up to two weeks using a centrifugal blood pump. Data were compared to prebypass control values and to results obtained from seven calves that underwent nonthoracotomy, retrograde LV cannulation, and pulsatile bypass for up to 35 days. Nonpulsatile flow produced an increase in heart rate of 20% ( P P ( Arch Surg 111:1225-1230, 1976)

Prolonged Pulseless Perfusion in Unanesthetized Calves

https://onlinelibrary.wiley.com/doi/pdf/10.1034/j.1600-6143.2003.00134.x

NOS: the underlying mechanism preserving vascular integrity and during ex vivo warm kidney perfusion.

Pulseless cardiopulmonary bypass.

SUMMARY Dog kidneys under hypothermia were perfused with canine plasma or human albumin as perfusion medium for 90 hr and thereafter autotransplanted. During preservation, samples were taken at regular intervals from the perfusate, and the lactate and pyruvate concentrations were measured. A rising lactate to pyruvate ratio, explained by the accumulation of toxic metabolic products in the perfusate, was observed after 24 hr of perfusion; at this stage the perfusate was therefore exchanged. Successful 96-hr preservation was possible, as could be demonstrated by p-aminohippuric acid and inulin clearances and by the serum creatinine level after transplantation. Repeated perfusate exchange, however, did not enable further prolongation of the preservation period. Furthermore, successful 96-hr preservation was likewise possible when the perfusate volume was doubled and the perfusate was not exchanged. The conclusion is that for long-term kidney preservation a human albumin solution with high protein and normal sodium content should be used, that the perfusate volume should amount to at least 1,200 ml, and that perfusate exchange should be carried out after 24 hr of perfusion.

Ninety-six-hour preservation of canine kidneys.

Nonpulsatile vs. pulsatile canine kidney perfusion.

Die maschinelle hypotherme Dauerperfusion stellt das Verfahren der Wahl dar, Nieren fur mindestens 72 Stunden erfolgreich zu konservieren. Dieser Methode, wie sie ursprunglich von BELZER (1) beschrieben wurde, haften jedoch einige entscheidende Nachteile an: 1. Als Perfusat wird cryoprazipitiertes Plasma benutzt, dessen Zubereitung (Gefrieren, Auftauen, Sterilfiltration) aufwendig und zeitraubend ist und bei dessen Verwendung immer mit einem Hepatitisrisiko zu rechnen ist. 2. Zur Oxygenierung wird neben Zimmerluft Sauerstoff verwendet, der pH wird durch zusatzliche CO2-Zufuhr konstant gehalten. 3. Das Gerat ist unhandlich, schwer und teuer.

Berr F

Papers

Ninety-six-hour preservation of canine kidneys.

Nonpulsatile vs. pulsatile canine kidney perfusion.

Erfolgreiche 72-Stunden Konservierung von Hundenieren durch hypotherme nicht-pulsierende Perfusion

[Comparative studies on pulsating and non-pulsating perfused canine kidney].