https://cyberleninka.org/article/n/876404.pdf

ECM and ECM-like materials – Biomaterials for applications in regenerative medicine and cancer therapy

Electrospinning versus microfluidic spinning of functional fibers for biomedical applications.

A two-pronged strategy of biomechanically active and biochemically functional hydrogel wound dressing which can assist wound closure and have multiple functions to promote wound healing has been ra...

Two-Pronged Strategy of Biomechanically Active and Biochemically Multifunctional Hydrogel Wound Dressing To Accelerate Wound Closure and Wound Healing

Electrospun polymeric nanofibres as wound dressings: A review.

The tumor microenvironment (TME) is comprised of many different cell populations, such as cancer-associated fibroblasts and various infiltrating immune cells, and non-cell components of extracellular matrix. These crucial parts of the surrounding stroma can function as both positive and negative regulators of all hallmarks of cancer development, including evasion of apoptosis, induction of angiogenesis, deregulation of the energy metabolism, resistance to the immune detection and destruction, and activation of invasion and metastasis. This review represents a summary of recent studies focusing on describing these effects of microenvironment on initiation and progression of the head and neck squamous cell carcinoma, focusing on oral squamous cell carcinoma, since it is becoming clear that an investigation of differences in stromal composition of the head and neck squamous cell carcinoma microenvironment and their impact on cancer development and progression may help better understand the mechanisms behind different responses to therapy and help define possible targets for clinical intervention.

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Effect of tumor microenvironment on pathogenesis of the head and neck squamous cell carcinoma: a systematic review

Biomimetic LBL structured nanofibrous matrices assembled by chitosan/collagen for promoting wound healing

Wound-healing improvement by resurfacing split-thickness skin donor sites with thin split-thickness grafting

The invention discloses intelligent wireless sensing dressing capable of being used for monitoring wound surface heeling in real time. The intelligent wireless sensing dressing comprises wound surface dressing and a monitoring device, wherein the monitoring device is embedded in the wound surface dressing, is used for monitoring the temperature, the humidity, the pH value, the oxygen content, the NO (Nitrogen Oxide) concentration and microbes of the wound surface, and comprises a CPU (Central Processing Unit) control module, an input module, an output module, a Bluetooth module, sensor modules, a storage module and a power supply module; the CPU module is arranged on a flexible circuit board or an ordinary circuit board; the input module, the output module, the Bluetooth module, the sensor module, the storage module and the power supply module are interacted with the CPU control module; and the power supply module is simultaneously and electrically connected with the input module, the output module, the Bluetooth module, the storage module and various sensor modules. The intelligent wireless sensing dressing has the advantages that the wound surface information can be provided under the condition without changing the dressing; the dressing is reasonably changed according to the heeling condition of a patient; and the personalized medical care is realized, so that unnecessary waste is reduced; the pain of the patient is reduced; the wound surface heeling is accelerated; the hospital staying time of the patient is shortened; and the hospitalization expenses are reduced.

Intelligent wireless sensing dressing capable of being used for monitoring wound surface heeling in real time

Purposes 
To compare the biological behaviors of human adipose-derived stem cells (ADSCs) isolated from adipose tissue by lipectomy and liposuction, with the purpose of providing the basis for clinical application.
Methods 
The proliferation and apoptosis of ADSCs were analyzed by CCK-8 assay and flow cytometry. Cell migration was measured by a wound healing assay. An ELISA assay was used to evaluate paracrine functions. SOD and MDA were tested by xanthine oxidase and thiobarbituric acid reactions, respectively. In addition, we used a CCK-8, LDH assay and flow cytometry to analyze the proliferation and apoptosis of ADSCs treated with lidocaine or adrenaline.
Results 
The viable ADSCs yield from liposuction was significantly lower than that from lipectomy, while the apoptosis of cells from liposuction was significantly higher than from lipectomy. The paracrine secretion of the two sources of ADSCs was highest when treated with 10−7 mol/L insulin and 10 ng/mL TGF-α, but there were no significant differences in VEGF, IL-6, IL-8 or HGF levels. The ADSCs from lipectomy migrated faster than those from liposuction, and SOD in the lipectomy group was higher than in the liposuction group, whereas MDA of the lipectomy group was lower than that of the liposuction group. The proliferation ADSCs treated with lidocaine or adrenaline was greatly decreased, while apoptosis was significantly increased, and cytotoxicity of lidocaine or adrenaline to ADSCs was dose-dependent.
Conclusions 
Compared with ADSCs from liposuction, the ADSCs from lipectomy have better biological characteristics. Lidocaine and adrenaline decreased the viability of ADSCs, and their cytotoxicity to ADSCs was dose-dependent.

/pdf/a-comparative-study-on-the-biological-characteristics-of-1n80jyf5tx.pdf

A Comparative Study on the Biological Characteristics of Human Adipose-Derived Stem Cells from Lipectomy and Liposuction

Developing basement membranes (BMs) substitute remains major problem for constructing functional tissue engineered skin because of its complex structure and multifunction of regulating cellular behavior. Herein, a stable electrospinning method was employed to generate a biomimetic model of natural BMs based on novel scaffold electrospun from Poly(ɛ-caprolactone) (PCL) and cellulose acetate (CA) incorporated with chitosan (CS). The morphology, structure, surface hydrophilicity, roughness and mechanical tensile strength of prepared monolayer and tri-layered scaffold were comprehensively compared. Besides, co-culture system via seeding keratinocytes (Kcs) and fibroblasts (Fbs) on opposite side of tri-layered scaffold revealed more effective segregation of both cell types within the central nanofibrous barrier together with enhanced cell attachment and proliferation than that on the monolayer scaffold. Moreover, the deposition of type VII collagen and laminin-5 was examined in comparison with normal skin BMs. Furthermore, the histological studies revealed characteristics of reconstructing BM zone at the junction of dermis-epidermis after in vivo implantation for 2 weeks, and wound healing while the seeded cells interacted with the endogenous cells. Additionally, the expression of active integrin β1 and phosphorylated focal adhesion kinase (FAK) was promoted with treatment of tri-layered scaffold. This study stressed that this tri-layer scaffold might provoke biomimetic responses of Kcs and Fbs and thus be applied for future development of BMs containing tissues.

Bian Yongqian

Papers

Biomimetic LBL structured nanofibrous matrices assembled by chitosan/collagen for promoting wound healing

Wound-healing improvement by resurfacing split-thickness skin donor sites with thin split-thickness grafting

Intelligent wireless sensing dressing capable of being used for monitoring wound surface heeling in real time

A Comparative Study on the Biological Characteristics of Human Adipose-Derived Stem Cells from Lipectomy and Liposuction

A Biomimetic Basement Membrane Substitute Based on Tri-Layered Nanofibrous Scaffold for Skin Reconstruction.